Overview

  • Product name
  • Description
    Rabbit polyclonal to CPSF2
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residue 450 and 500 of Human CPSF2 (NP_059133.1).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab81553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 88 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    CPSF2 belongs to the RNA-metabolizing metallo-beta-lactamase-like family, CPSF2/YSH1 subfamily. It is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3 prime-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. A poly(A) tail is added to eukaryotic mRNA precursors after tightly coupled cleavage and polyadenylation reactions at their 3-prime ends.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Cleavage and polyadenylation specific factor 2 antibody
    • cleavage and polyadenylation specific factor 2, 100kDa antibody
    • Cleavage and polyadenylation specificity factor 100 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 2 antibody
    • CPSF 100 kDa subunit antibody
    • CPSF 2 antibody
    • CPSF100 antibody
    • KIAA1367 antibody
    • zgc:92484 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic islet cell tumor (left) and mouse teratoma (right) tissues labelling CPSF2 with ab81553 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CPSF2 antibody (ab81553) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • HeLa cell lysate (1mg) was immunoprecipitated using ab81553 at 3µg/mg (lane 1) or IgG control (lane 2). 20% of the immunoprecipitate was analysed by Western blotting using ab81553 at 1µg/ml. Bands were observed by chemiluminescence with a 3 second exposure time.

References

ab81553 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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