Overview

  • Product nameAnti-CPSF2 antibody
    See all CPSF2 primary antibodies
  • Description
    Rabbit polyclonal to CPSF2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide designed between residues 732 and 782 of Human CPSF2 (NP_059133.1)

  • Positive control
    • HeLa, 293T or NIH3T3 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 88 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceCPSF2 belongs to the RNA-metabolizing metallo-beta-lactamase-like family, CPSF2/YSH1 subfamily. It is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3 prime-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. A poly(A) tail is added to eukaryotic mRNA precursors after tightly coupled cleavage and polyadenylation reactions at their 3-prime ends.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Cleavage and polyadenylation specific factor 2 antibody
    • cleavage and polyadenylation specific factor 2, 100kDa antibody
    • Cleavage and polyadenylation specificity factor 100 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 2 antibody
    • CPSF 100 kDa subunit antibody
    • CPSF 2 antibody
    • CPSF100 antibody
    • KIAA1367 antibody
    • zgc:92484 antibody
    see all

Anti-CPSF2 antibody images

  • All lanes : Anti-CPSF2 antibody (ab81554) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 seconds
  • HeLa whole cell lysate (1mg) was immunoprecipitated with ab81554 at 3µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab81554 at 1µg/ml.

References for Anti-CPSF2 antibody (ab81554)

ab81554 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"