CPSF2 belongs to the RNA-metabolizing metallo-beta-lactamase-like family, CPSF2/YSH1 subfamily. It is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3 prime-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. A poly(A) tail is added to eukaryotic mRNA precursors after tightly coupled cleavage and polyadenylation reactions at their 3-prime ends.
Cleavage and polyadenylation specific factor 2 antibody
cleavage and polyadenylation specific factor 2, 100kDa antibody
Cleavage and polyadenylation specificity factor 100 kDa subunit antibody
Cleavage and polyadenylation specificity factor subunit 2 antibody
CPSF 100 kDa subunit antibody
CPSF 2 antibody
Western blot - CPSF2 antibody (ab81554)
All lanes : Anti-CPSF2 antibody (ab81554) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 88 kDa Observed band size : 88 kDa Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 seconds
Immunoprecipitation - CPSF2 antibody (ab81554)
HeLa whole cell lysate (1mg) was immunoprecipitated with ab81554 at 3µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab81554 at 1µg/ml.