Overview

  • Product name
    Anti-CPSF2 antibody [EPR7599]
    See all CPSF2 primary antibodies
  • Description
    Rabbit monoclonal [EPR7599] to CPSF2
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human CPSF2.

  • Positive control
    • HeLa, Ramos, Jurkat, and 293T cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 88 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Relevance
      CPSF2 belongs to the RNA-metabolizing metallo-beta-lactamase-like family, CPSF2/YSH1 subfamily. It is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3 prime-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. A poly(A) tail is added to eukaryotic mRNA precursors after tightly coupled cleavage and polyadenylation reactions at their 3-prime ends.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • Cleavage and polyadenylation specific factor 2 antibody
      • cleavage and polyadenylation specific factor 2, 100kDa antibody
      • Cleavage and polyadenylation specificity factor 100 kDa subunit antibody
      • Cleavage and polyadenylation specificity factor subunit 2 antibody
      • CPSF 100 kDa subunit antibody
      • CPSF 2 antibody
      • CPSF100 antibody
      • KIAA1367 antibody
      • zgc:92484 antibody
      see all

    Images

    • All lanes : Anti-CPSF2 antibody [EPR7599] (ab126760) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 88 kDa

    References

    This product has been referenced in:
    • Guillén-Boixet J  et al. CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets. Elife 5:N/A (2016). Read more (PubMed: 27802129) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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