Recombinant Anti-CPSF30/NAR antibody [EPR7433] (ab131218)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7433] to CPSF30/NAR
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CPSF30/NAR antibody [EPR7433]
See all CPSF30/NAR primary antibodies -
Description
Rabbit monoclonal [EPR7433] to CPSF30/NAR -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CPSF30/NAR aa 1-100. The exact sequence is proprietary.
Database link: O95639 -
Positive control
- Jurkat, 293T, and HepG2 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.69 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7433 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa. |
Target
-
Function
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U). -
Sequence similarities
Belongs to the CPSF4/YTH1 family.
Contains 5 C3H1-type zinc fingers.
Contains 1 CCHC-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 10898 Human
- Entrez Gene: 54188 Mouse
- Entrez Gene: 304277 Rat
- Omim: 603052 Human
- SwissProt: O95639 Human
- SwissProt: Q8BQZ5 Mouse
- SwissProt: Q5FVR7 Rat
- Unigene: 489287 Human
see all -
Alternative names
- cleavage and polyadenylation specific factor 4, 30kDa antibody
- Cleavage and polyadenylation specificity factor 30 kDa subunit antibody
- Cleavage and polyadenylation specificity factor subunit 4 antibody
see all
Images
-
All lanes : Anti-CPSF30/NAR antibody [EPR7433] (ab131218) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 30 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab131218 has not yet been referenced specifically in any publications.