• Product nameAnti-CPSF73 antibody
    See all CPSF73 primary antibodies
  • Description
    Rabbit polyclonal to CPSF73
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: RLEIMLQDIF GEDCVSVKDD SILSVTVDGK TANLNLETRT VECEEGSEDD, corresponding to a region within C terminal amino acids 613-662 of Human CPSF73

  • Positive control
    • Jurkat cell lysate. Human liver tissue.



Our Abpromise guarantee covers the use of ab66671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 77 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.


  • FunctionComponent of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.
  • Sequence similaritiesBelongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
  • Post-translational
    Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cleavage and polyadenylation specific factor 3 73kDa antibody
    • Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 3 antibody
    • CPSF 73 antibody
    • CPSF 73 kDa subunit antibody
    • CPSF antibody
    • CPSF-73 antibody
    • cpsf3 antibody
    • CPSF3_HUMAN antibody
    • CPSF73 antibody
    • mRNA 3''-end-processing endonuclease CPSF-73 antibody
    • YSH1 antibody
    see all

Anti-CPSF73 antibody images

  • All lanes : Anti-CPSF73 antibody (ab66671) at 1.25 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CPSF73 with ab66671 at 4-8µg/ml. Arrows indicate positively labelled liver cells. Magnification: 400X.

References for Anti-CPSF73 antibody (ab66671)

ab66671 has not yet been referenced specifically in any publications.

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