• Product nameAnti-CPSF73 antibody
    See all CPSF73 primary antibodies
  • Description
    Rabbit polyclonal to CPSF73
  • Tested applicationsSuitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region between residue 1 and 50 of human CPSF73 (NP_057291.1)

  • Positive control
    • Whole cell lysates from HeLa, 293T and mouse NIH3T3 cells.



Our Abpromise guarantee covers the use of ab72294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionComponent of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.
    • Sequence similaritiesBelongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
    • Post-translational
      Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cleavage and polyadenylation specific factor 3 73kDa antibody
      • Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
      • Cleavage and polyadenylation specificity factor subunit 3 antibody
      • CPSF 73 antibody
      • CPSF 73 kDa subunit antibody
      • CPSF antibody
      • CPSF-73 antibody
      • cpsf3 antibody
      • CPSF3_HUMAN antibody
      • CPSF73 antibody
      • mRNA 3''-end-processing endonuclease CPSF-73 antibody
      • YSH1 antibody
      see all

    Anti-CPSF73 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CPSF73 with ab72294 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-CPSF73 antibody (ab72294) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 5 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

      developed using the ECL technique

      Predicted band size : 77 kDa
      Observed band size : 77 kDa

      Exposure time : 3 minutes

    References for Anti-CPSF73 antibody (ab72294)

    ab72294 has not yet been referenced specifically in any publications.

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