Anti-CPSF73 antibody (ab72295)
Key features and details
- Rabbit polyclonal to CPSF73
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-CPSF73 antibody
See all CPSF73 primary antibodies -
Description
Rabbit polyclonal to CPSF73 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human CPSF73.
Database link: Q9UKF6 -
Positive control
- WB: HeLa, HEK293T, NIH 3T3. IHC-P: human breast carcinoma, mouse teratoma tissues. IP: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab72295 was affinity purified using an epitope specific to CPSF73 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (5) |
1/2000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
|
IP | (2) |
Use at 2-5 µg/mg of lysate.
|
IHC-P |
1/200.
|
Notes |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200. |
Target
-
Function
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. -
Sequence similarities
Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily. -
Post-translational
modificationsSumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 281712 Cow
- Entrez Gene: 51692 Human
- Entrez Gene: 54451 Mouse
- Omim: 606029 Human
- SwissProt: P79101 Cow
- SwissProt: Q9UKF6 Human
- SwissProt: Q9QXK7 Mouse
- Unigene: 515972 Human
see all -
Alternative names
- Cleavage and polyadenylation specific factor 3 73kDa antibody
- Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
- Cleavage and polyadenylation specificity factor subunit 3 antibody
see all
Images
-
All lanes : Anti-CPSF73 antibody (ab72295) at 0.04 µg/ml
Lane 1 : HeLa whole cell
lysate at 50 µg
Lane 2 : HeLa whole cell
lysate at 15 µg
Lane 3 : HeLa whole cell
lysate at 5 µg
Lane 4 : 293T whole cell
lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell
lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling CPSF73 with ab72295 at 1/200 (1µg/ml). Detection: DAB.
-
Detection of CPSF73 by Western Blot of Immunprecipitate. Lane 1: ab72295, at 0.1µg/ml, staining CPSF73 in HeLa whole cell lysate immunoprecipitated using ab72225 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).br> Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab72295 has been referenced in 4 publications.
- Arnadottir GA et al. Population-level deficit of homozygosity unveils CPSF3 as an intellectual disability syndrome gene. Nat Commun 13:705 (2022). PubMed: 35121750
- Eaton JD et al. Xrn2 accelerates termination by RNA polymerase II, which is underpinned by CPSF73 activity. Genes Dev 32:127-139 (2018). PubMed: 29432121
- Cardiello JF et al. Heat Shock Causes a Reversible Increase in RNA Polymerase II Occupancy Downstream of mRNA Genes, Consistent with a Global Loss in Transcriptional Termination. Mol Cell Biol 38:N/A (2018). PubMed: 29967245
- Banerjee A et al. Nuclear poly(A) binding protein 1 (PABPN1) and Matrin3 interact in muscle cells and regulate RNA processing. Nucleic Acids Res 45:10706-10725 (2017). WB . PubMed: 28977530