Overview

  • Product nameAnti-CPSF73 antibody
    See all CPSF73 primary antibodies
  • Description
    Rabbit polyclonal to CPSF73
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide correspondig to a region between residues 634 and 684 of human CPSF73 (NP_057291.1)

  • Positive control
    • HeLa, 293T and mouse NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab72295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionComponent of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.
  • Sequence similaritiesBelongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
  • Post-translational
    modifications
    Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cleavage and polyadenylation specific factor 3 73kDa antibody
    • Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 3 antibody
    • CPSF 73 antibody
    • CPSF 73 kDa subunit antibody
    • CPSF antibody
    • CPSF-73 antibody
    • cpsf3 antibody
    • CPSF3_HUMAN antibody
    • CPSF73 antibody
    • mRNA 3''-end-processing endonuclease CPSF-73 antibody
    • YSH1 antibody
    see all

Anti-CPSF73 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling CPSF73 with ab72295 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CPSF73 antibody (ab72295) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 77 kDa
    Observed band size : 77 kDa


    Exposure time : 30 seconds
  • Detection of CPSF73 by Western Blot of Immunprecipitate. Lane 1: ab72295, at 0.1µg/ml, staining CPSF73 in HeLa whole cell lysate immunoprecipitated using ab72225 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).br> Lane 2: Control IgG.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-CPSF73 antibody (ab72295)

ab72295 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela cells)
Loading amount 100000 cells
Specification Hela cells
Gel Running Conditions Non-reduced Denaturing (NuPage 4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted May 14 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cells)
Specification Hela cells
Fixative Methanol
Permeabilization No
Blocking step Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
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Submitted Apr 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse ES cells)
Loading amount 50000 cells
Specification mouse ES cells
Treatment 72 hours after esiRNA Knockdown
Gel Running Conditions Non-reduced Denaturing (Invitrogen NuPage 4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Apr 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"