Overview

  • Product nameAnti-CPT1A antibody
    See all CPT1A primary antibodies
  • Description
    Goat polyclonal to CPT1A
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to amino acids 621-634 (

    C-DPAQTVEQRLKLFK

    ) from an internal region of Human CPT1A (NP_001027017.1).

  • Positive control
    • Human liver lysate; Human heart and kidney tissues

Applications

Our Abpromise guarantee covers the use of ab115269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 88 kDa.
ELISA 1/128000.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificityStrong expression in kidney and heart, and lower in liver and skeletal muscle.
  • PathwayLipid metabolism; fatty acid beta-oxidation.
  • Involvement in diseaseDefects in CPT1A are the cause of carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]; also known as CPT-I deficiency or CPT1A deficiency. CPT1AD is a rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood.
  • Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
  • Cellular localizationMitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carnitine O palmitoyltransferase 1 liver isoform antibody
    • Carnitine O palmitoyltransferase I antibody
    • Carnitine O palmitoyltransferase I liver isoform antibody
    • Carnitine O-palmitoyltransferase 1 antibody
    • Carnitine O-palmitoyltransferase I antibody
    • Carnitine palmitoyltransferase 1A (liver) antibody
    • Carnitine palmitoyltransferase 1A antibody
    • Carnitine palmitoyltransferase I antibody
    • Carnitine palmitoyltransferase I liver antibody
    • CPT 1 antibody
    • CPT I antibody
    • CPT1 antibody
    • CPT1 L antibody
    • CPT1-L antibody
    • Cpt1a antibody
    • CPT1A_HUMAN antibody
    • CPTI antibody
    • CPTI-L antibody
    • L CPT1 antibody
    • liver isoform antibody
    see all

Anti-CPT1A antibody images

  • Anti-CPT1A antibody (ab115269) at 0.1 µg/ml + Human liver lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 88 kDa


    Exposure time : 1 hour
  • ab115269, at 4 ug/ml, staining CPT1A in formalin fixed, paraffin embedded Human heart tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated anti-goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab115269, at 4 ug/ml, staining CPT1A in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated anti-goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-CPT1A antibody (ab115269)

ab115269 has not yet been referenced specifically in any publications.

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