Overview

  • Product name
  • Description
    Rabbit polyclonal to CPT1A
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 684-733 (LSTSQTPQQQ VELFDLENNP EYVSSGGGFG PVADDGYGVS YILVGENLIN) of human CPT1A (NP_001027017).

  • Positive control
    • DU145 cell line IF/ICC 293T cell lysate

Applications

Our Abpromise guarantee covers the use of ab83862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 88 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Tissue specificity
    Strong expression in kidney and heart, and lower in liver and skeletal muscle.
  • Pathway
    Lipid metabolism; fatty acid beta-oxidation.
  • Involvement in disease
    Defects in CPT1A are the cause of carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]; also known as CPT-I deficiency or CPT1A deficiency. CPT1AD is a rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood.
  • Sequence similarities
    Belongs to the carnitine/choline acetyltransferase family.
  • Cellular localization
    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carnitine O palmitoyltransferase 1 liver isoform antibody
    • Carnitine O palmitoyltransferase I antibody
    • Carnitine O palmitoyltransferase I liver isoform antibody
    • Carnitine O-palmitoyltransferase 1 antibody
    • Carnitine O-palmitoyltransferase I antibody
    • Carnitine palmitoyltransferase 1A (liver) antibody
    • Carnitine palmitoyltransferase 1A antibody
    • Carnitine palmitoyltransferase I antibody
    • Carnitine palmitoyltransferase I liver antibody
    • CPT 1 antibody
    • CPT I antibody
    • CPT1 antibody
    • CPT1 L antibody
    • CPT1-L antibody
    • Cpt1a antibody
    • CPT1A_HUMAN antibody
    • CPTI antibody
    • CPTI-L antibody
    • L CPT1 antibody
    • liver isoform antibody
    see all

Anti-CPT1A antibody images



  • Predicted band size : 88 kDa
    Western blot analysis of Capan1 (lane 1) and HPAF (lane 2) cell lysates (45µg/lane) labeling CPT1A with ab83862 at 1:1000. A HRP-conjugated anti-rabbit (1:5000) was used as the secondary antibody.
  • Anti-CPT1A antibody (ab83862) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
    12% gel concentration
  • ICC/IF image of ab83862 stained DU145 cells. The cells were 4% formladehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83862, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Sample Type : Human Capan1 cells (Pancreatic cancer cell line). Primary Antibody Dilution : 1:300. Secondary Antibody : Anti-rabbit-AlexaFluor-488. Secondary Antibody Dilution : 1:200. Color/Signal Descriptions : CPT1A: Green DAPI: Blue. Gene Name : CPT1A.

References for Anti-CPT1A antibody (ab83862)

This product has been referenced in:
  • Wagschal A  et al. Genome-wide identification of microRNAs regulating cholesterol and triglyceride homeostasis. Nat Med 21:1290-7 (2015). Read more (PubMed: 26501192) »
  • Chen LY  et al. Promotion of mitochondrial energy metabolism during hepatocyte apoptosis in a rat model of acute liver failure. Mol Med Rep 12:5035-41 (2015). WB ; Rat . Read more (PubMed: 26135512) »

See all 2 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (7.5 %)
Loading amount
15 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 05 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Cow Cell lysate - whole cell (fetal liver, cumulus oocyte complexes)
Specification
fetal liver, cumulus oocyte complexes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Leni Van Tol

Verified customer

Submitted Jul 11 2014

DISCOUNT CODE:***
Expiration date: July 13th, 2012
I am very pleased to hear you would like to test ab83862 in monkey. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (Liver)
Loading amount
40 µg
Specification
Liver
Treatment
Alcohol
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 28 2012

DISCOUNT CODE: *** Expiration date: June 22nd, 2012

I am very pleased to hear you would like to accept our offer and test ab83862 in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, ple...

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