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Synthetic peptide: DIADLFQVPKAYS, corresponding to C terminal amino acids 760-772 of Human CPT1B.
Our Abpromise guarantee covers the use of ab15703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3 - 5 µg/ml.|
|WB||Use at an assay dependent dilution. Can be blocked with Human CPT1B peptide (ab23261). Preliminary experiments gave no signal but low background in human kidney and HepG2 lysates at up to 1µg/ml. We would appreciate any feedback from customers.|
ab15703 at 3.8µg/ml staining human heart tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP-staining procedure was used for detection.
ab15703 has not yet been referenced specifically in any publications.