The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May be a regulator of cytoskeletal organization. May have a role in spermatogenesis.
Belongs to the verprolin family. Contains 1 WH2 domain.
The WH2 domain is found in a number of putative actin-binding proteins. The profilin-binding motif has been implicated in the interaction with profilin and SH3 domains. The KLKR motif is essential for G-actin binding and for actin polymerization.
Cytoplasm. In hippocampal neurons colocalizes with WASL in the cell body, axons and the growth cone.