The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
FunctionMay be a regulator of cytoskeletal organization. May have a role in spermatogenesis.
Sequence similaritiesBelongs to the verprolin family. Contains 1 WH2 domain.
DomainThe WH2 domain is found in a number of putative actin-binding proteins. The profilin-binding motif has been implicated in the interaction with profilin and SH3 domains. The KLKR motif is essential for G-actin binding and for actin polymerization.
Cellular localizationCytoplasm. In hippocampal neurons colocalizes with WASL in the cell body, axons and the growth cone.