Overview

  • Product name
  • Description
    Rabbit polyclonal to CR16
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    ELSSKSQQAT AWTPTQQPGG QLRNGSLHII DDFESKFTFH SVEDFPPPDE YKPCQKIYPS, corresponding to amino acids 387-446 of Human CR16 (A6NGB9).

  • Positive control
    • Human testis lysate. U-2 OS cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • Function
    May be a regulator of cytoskeletal organization. May have a role in spermatogenesis.
  • Sequence similarities
    Belongs to the verprolin family.
    Contains 1 WH2 domain.
  • Domain
    The WH2 domain is found in a number of putative actin-binding proteins.
    The profilin-binding motif has been implicated in the interaction with profilin and SH3 domains.
    The KLKR motif is essential for G-actin binding and for actin polymerization.
  • Cellular localization
    Cytoplasm. In hippocampal neurons colocalizes with WASL in the cell body, axons and the growth cone.
  • Information by UniProt
  • Database links
  • Alternative names
    • Corticosteroids and regional expression protein 16 homolog antibody
    • WAS/WASL-interacting protein family member 3 antibody
    • Wipf3 antibody
    • WIPF3_HUMAN antibody

Images

  • ab122703 1/50 dilution, staining CR16 in Paraffin-embedded Human testis tissue by Immunohistochemistry
  • Immunofluorescent staining of Human cell line U-2 OS, using ab122703 at 4µg/ml. Antibody staining is shown in green.

References

ab122703 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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