Recombinant
RabMAb

Anti-CR1L antibody [EPR9604(B)] (ab169543)

Overview

  • Product name
    Anti-CR1L antibody [EPR9604(B)]
  • Description
    Rabbit monoclonal [EPR9604(B)] to CR1L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human CR1L (UniProt: Q2VPA4).

  • Positive control
    • HepG2, Ramos, K562 and MOLT4 cell lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9604(B)
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab169543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Relevance
      Interacts with iC4 (methylamine-treated C4) but not for iC3 (methylamine-treated C3). Belongs to the receptors of complement activation (RCA) family. Contains 8 Sushi (CCP/SCR) domains.
    • Cellular localization
      Cytoplasm. Membrane. Secreted. Note: Predominantly found in association with the membrane fraction, but also located in the cytoplasm and in the supernatant.
    • Database links
    • Alternative names
      • Complement C4b-binding protein CR-1-like protein antibody
      • Complement component receptor 1-like protein antibody
      • CR1L antibody

    Images

    • All lanes : Anti-CR1L antibody [EPR9604(B)] (ab169543) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 63 kDa

    • Flow Cytometric analysis of permeabilized HepG2 cells labeling CR1L with ab169543 at 1/100 dilution (red) compared to a rabbit IgG (negative) (green).

    References

    ab169543 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169543.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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