Overview

  • Product nameAnti-CRALBP antibody [B2]
    See all CRALBP primary antibodies
  • Description
    Mouse monoclonal [B2] to CRALBP
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig, Monkey
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: rat eye; rat retina.
  • General notes

    Alternative versions available:

    Anti-CRALBP antibody (HRP) [B2] (ab199992)

Properties

Applications

Our Abpromise guarantee covers the use of ab15051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22892561
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionSoluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.
  • Tissue specificityRetina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina.
  • Involvement in diseaseDefects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
    Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
    Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.
  • Sequence similaritiesContains 1 CRAL-TRIO domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular retinaldehyde binding protein 1 antibody
    • Cellular retinaldehyde binding protein antibody
    • Cellular retinaldehyde-binding protein antibody
    • MGC3663 antibody
    • Retinaldehyde binding protein 1 antibody
    • Retinaldehyde-binding protein 1 antibody
    • RLBP 1 antibody
    • RLBP1 antibody
    • RLBP1_HUMAN antibody
    see all

Anti-CRALBP antibody [B2] images

  • All lanes : Anti-CRALBP antibody [B2] (ab15051) at 1 µg/ml

    Lane 1 : Rat eye tissue lysate - total protein (ab4035)
    Lane 2 : Rat retina tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Immunofluorescence analysis of hESC-RPE monolayers, staining CRALBP (green) with ab15051.

    Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour. Cells were incubated with primary antibody (1/1000) for 1 hour at room temperature. An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/800) was used as the secondary antibody.
  • ab15051 staining rat retina sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab15051 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/250 was used as the secondary antibody. Müller cells in the eye retina are positive. Two different areas of the retina are shown.

    See Abreview

References for Anti-CRALBP antibody [B2] (ab15051)

This product has been referenced in:
  • Chung SH  et al. Differential expression of microRNAs in retinal vasculopathy caused by selective Müller cell disruption. Sci Rep 6:28993 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27373709) »
  • Rodriguez-Crespo D  et al. Triple-layered mixed co-culture model of RPE cells with neuroretina for evaluating the neuroprotective effects of adipose-MSCs. Cell Tissue Res 358:705-16 (2014). Read more (PubMed: 25213807) »

See all 22 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample Human Cell (iPSC-derived RPE cells)
Specification iPSC-derived RPE cells
Permeabilization Yes - 0.2% Triton
Fixative Paraformaldehyde
Username

Dr. Kathryn Davidson

Verified customer

Submitted Jan 23 2015

Application Immunohistochemistry (Frozen sections)
Sample Rhesus monkey Tissue sections (Retina)
Specification Retina
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 08 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Zebrafish Tissue sections (Retina)
Permeabilization No
Specification Retina
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

Submitted Jul 30 2013

As requested, I have issued a free of charge replacement with the order number XXXXX for the antibody ab15051 from a different lot to the last one received.

To check the status of the order please contact our Customer Service team and referen...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Retina)
Specification Retina
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jun 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (Neuroretina)
Specification Neuroretina
Fixative Paraformaldehyde
Permeabilization Yes - Triton x100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. I Fernandez-Bueno

Verified customer

Submitted Apr 07 2009

1-10 of 11 Abreviews or Q&A

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