Anti-CRALBP antibody [B2] (ab15051)
Key features and details
- Mouse monoclonal [B2] to CRALBP
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CRALBP antibody [B2]
See all CRALBP primary antibodies -
Description
Mouse monoclonal [B2] to CRALBP -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human, Pig, Monkey -
Immunogen
Recombinant full length protein corresponding to Human CRALBP.
Database link: P12271 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: rat eye; rat retina. FFPE Mouse eye tissue sections.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
B2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
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Notes |
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IHC-P
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'. -
Tissue specificity
Retina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina. -
Involvement in disease
Defects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments. -
Sequence similarities
Contains 1 CRAL-TRIO domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 282038 Cow
- Entrez Gene: 6017 Human
- Entrez Gene: 19771 Mouse
- Entrez Gene: 100521278 Pig
- Entrez Gene: 293049 Rat
- Omim: 180090 Human
- SwissProt: P10123 Cow
- SwissProt: P12271 Human
see all -
Alternative names
- Cellular retinaldehyde binding protein 1 antibody
- Cellular retinaldehyde binding protein antibody
- Cellular retinaldehyde-binding protein antibody
see all
Images
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Immunohistochemistry image of CRALBP staining in a section of formalin-fixed paraffin-embedded normal mouse eye performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15051, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : Anti-CRALBP antibody [B2] (ab15051) at 1 µg/ml
Lane 1 :Rat eye tissue lysate - total protein (ab4035)
Lane 2 : Rat retina tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (98)
ab15051 has been referenced in 98 publications.
- Bonilla-Pons SÀ et al. Müller glia fused with adult stem cells undergo neural differentiation in human retinal models. EBioMedicine 77:103914 (2022). PubMed: 35278743
- Dorgau B et al. Human Retinal Organoids Provide a Suitable Tool for Toxicological Investigations: A Comprehensive Validation Using Drugs and Compounds Affecting the Retina. Stem Cells Transl Med 11:159-177 (2022). PubMed: 35298655
- Palfi A et al. AAV-PHP.eB transduces both the inner and outer retina with high efficacy in mice. Mol Ther Methods Clin Dev 25:236-249 (2022). PubMed: 35474956
- Zeng S et al. Inhibiting the activation of MAPK (ERK1/2) in stressed Müller cells prevents photoreceptor degeneration. Theranostics 12:6705-6722 (2022). PubMed: 36185611
- Tang J et al. Increased Mobile Zinc Regulates Retinal Ganglion Cell Survival via Activating Mitochondrial OMA1 and Integrated Stress Response. Antioxidants (Basel) 11:N/A (2022). PubMed: 36290724