Overview

  • Product name
  • Description
    Mouse polyclonal to CRBN
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human CRBN aa 1-442.
    Sequence:

    MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFD TSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQ LFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDF GIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLE SLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDA ETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLK IGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYV NPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGW KFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL


    Database link: Q96SW2

  • Positive control
    • WB: CRBN transfected 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • Function
    Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1.
  • Tissue specificity
    Widely expressed. Highly expressed in brain.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Defects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A) [MIM:607417]. MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • Sequence similarities
    Belongs to the CRBN family.
    Contains 1 Lon domain.
  • Post-translational
    modifications
    Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
  • Cellular localization
    Cytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610203G15Rik antibody
    • 2900045O07Rik antibody
    • AF229032 antibody
    • AW108261 antibody
    • Cereblon antibody
    • Crbn antibody
    • CRBN_HUMAN antibody
    • DKFZp781K0715 antibody
    • MGC27358 antibody
    • MRT2A antibody
    • OTTHUMP00000209555 antibody
    • piL antibody
    • Protein cereblon antibody
    • Protein x 0001 antibody
    see all

Anti-CRBN antibody images

  • All lanes : Anti-CRBN antibody (ab68763) at 1/500 dilution

    Lane 1 : CRBN transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate


    Predicted band size : 51 kDa

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

References for Anti-CRBN antibody (ab68763)

This product has been referenced in:
  • Gandhi AK  et al. Measuring cereblon as a biomarker of response or resistance to lenalidomide and pomalidomide requires use of standardized reagents and understanding of gene complexity. Br J Haematol 164:233-44 (2014). WB . Read more (PubMed: 24206017) »

See 1 Publication for this product

Product Wall

Application
Western blot
Sample
Mouse Purified protein (Purified protein of HEK293(mouse CRBN-His6 overexp)
Gel Running Conditions
Reduced Denaturing (10 % gel)
Loading amount
3 µg
Specification
Purified protein of HEK293(mouse CRBN-His6 overexp
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Treatment
CRBN Overexpression
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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