Anti-CRBN antibody (ab98992)

Overview

  • Product nameAnti-CRBN antibody
    See all CRBN primary antibodies
  • Description
    Rabbit polyclonal to CRBN
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 35-84 (DQDSKEAKKP NIINFDTSLP TSHTYLGADM EEFHGRTLHD DDSCQVIPVL) of Human CRBN (NP_057386).

  • Positive control
    • COLO205 cell IF/ICC: SKNSH cell line

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionComponent of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1.
  • Tissue specificityWidely expressed. Highly expressed in brain.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseDefects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A) [MIM:607417]. MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • Sequence similaritiesBelongs to the CRBN family.
    Contains 1 Lon domain.
  • Post-translational
    modifications
    Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
  • Cellular localizationCytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610203G15Rik antibody
    • 2900045O07Rik antibody
    • AF229032 antibody
    • AW108261 antibody
    • Cereblon antibody
    • Crbn antibody
    • CRBN_HUMAN antibody
    • DKFZp781K0715 antibody
    • MGC27358 antibody
    • MRT2A antibody
    • OTTHUMP00000209555 antibody
    • piL antibody
    • Protein cereblon antibody
    • Protein x 0001 antibody
    see all

Anti-CRBN antibody images

  • ICC/IF image of ab98992 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98992, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CRBN antibody (ab98992) at 1 µg/ml + COLO205 cell lysate at 10 µg

    Predicted band size : 50 kDa

References for Anti-CRBN antibody (ab98992)

ab98992 has not yet been referenced specifically in any publications.

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Expiration date: May 30th, 2012

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Abreviews
Application Western blot
Sample Mouse Purified protein (Purified protein of HEK293(mouse CRBN-His6 overexp)
Gel Running Conditions Reduced Denaturing (10 % gel)
Loading amount 3 µg
Specification Purified protein of HEK293(mouse CRBN-His6 overexp
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 30 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Myeloma cell)
Specification Myeloma cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Feb 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"