Anti-Cre recombinase antibody [7.23] (ab24607)

Overview

  • Product nameAnti-Cre recombinase antibody [7.23]
    See all Cre recombinase primary antibodies
  • Description
    Mouse monoclonal [7.23] to Cre recombinase
  • SpecificityCurrent Batch not validated for Immunohistochemistry.
  • Tested applicationsSuitable for: WBmore details
  • Immunogen

    Full length native protein (affinity purified).

  • General notes


    This antibody can be used to determine the success of gene manipulations in transgenic animals.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.03% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody can be used to determine the success of gene manipulations in transgenic animals.
  • ClonalityMonoclonal
  • Clone number7.23
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).

Target

  • RelevanceCre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • Alternative names
    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody

Anti-Cre recombinase antibody [7.23] images

  • All lanes : Anti-Cre recombinase antibody [7.23] (ab24607) at 1/1000 dilution

    Lane 1 : Escherichia coli expressing Cre-recombinase
    Lane 2 : Hek cell expressing control plasmid

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-mouse HRP conjugated at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50&80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This image is courtesy of Britta Eickholt, Univ London Kings Coll, United Kingdom

References for Anti-Cre recombinase antibody [7.23] (ab24607)

This product has been referenced in:
  • Wu Y  et al. Ga12 is required for renal cystogenesis induced by Pkd1 inactivation. J Cell Sci 129:3675-3684 (2016). Read more (PubMed: 27505895) »
  • Ding J & Delpire E Deletion of KCC3 in parvalbumin neurons leads to locomotor deficit in a conditional mouse model of peripheral neuropathy associated with agenesis of the corpus callosum. Behav Brain Res 274:128-36 (2014). Mouse . Read more (PubMed: 25116249) »

See all 9 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Rat Tissue sections (Brain tissue (Inferior colliculus and Medial Genic)
Specification Brain tissue (Inferior colliculus and Medial Genic
Username

Abcam user community

Verified customer

Submitted Jun 14 2017

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse embryonic spinal cord (cryosections))
Permeabilization Yes - 0.1%Triton X-100
Specification mouse embryonic spinal cord (cryosections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Dr. Frederic Clotman

Verified customer

Submitted Nov 19 2015

Thank you for contacting us.

Please check the following;

http://www.ncbi.nlm.nih.gov/pubmed/18408763?dopt=Abstract

It is also used in other publications but we are unable to confirm the applications due to non availability of...

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Ante todo quisiera desearle un muy Feliz Año.

Muchas gracias por tomarte la molestia de rellenar el cuestionario y mandar más imágenes.

Hay alguna sugerencia que posiblemente mejore la tinción obtenida. M...

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Thank you for contacting us.

Perhaps these are the antibodies customer is looking for;

http://www.abcam.com/cre-recombinase-antibody-723-ab24607.html

http://www.abcam.com/cre-recombinase-antibody-723-biotin-ab24580.html
<...

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Ich möchte Ihnen hiermit nochmals viel Erfolg wünschen und drücke die Daumen, dass dieser Antikörper funktioniert.

Wenn Sie Ihre Versuche abgeschlossen haben würden wir uns über einen Abreview freuen. Die gesammel...

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Vielen Dank für diese zusätzliche Information.

Leider haben wir keinen Antikörper den ich für die Detektion Ihres rekombinanten Proteins garantieren kann. Ich habe drei Antikörper gefunden die vielleicht für Sie i...

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Thank you for contacting us.

We have 2 antibodies in our catalogwhich have beentested in IHC-Fr: ab24607, ab24580 (biotinylated).

http://www.abcam.com/cre-recombinase-antibody-723-biotin-ab24580.html
http://www.abcam.com/cre-r...

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Thank you for contacting us.

Your credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask...

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Thank you for your reply.


I am happy to offer a replacement from another lot or a credit for this antibody. Please let me know which you would prefer.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"