Anti-Cre recombinase antibody [7.23] (ab24607)


  • Product name
    Anti-Cre recombinase antibody [7.23]
    See all Cre recombinase primary antibodies
  • Description
    Mouse monoclonal [7.23] to Cre recombinase
  • Specificity
    Current Batch not validated for Immunohistochemistry.
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Full length native protein (affinity purified).

  • General notes

    This antibody can be used to determine the success of gene manipulations in transgenic animals.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.03% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    This antibody can be used to determine the success of gene manipulations in transgenic animals.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab24607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).


  • Relevance
    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • Alternative names
    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody

Anti-Cre recombinase antibody [7.23] images

  • All lanes : Anti-Cre recombinase antibody [7.23] (ab24607) at 1/1000 dilution

    Lane 1 : Escherichia coli expressing Cre-recombinase
    Lane 2 : Hek cell expressing control plasmid

    Lysates/proteins at 10 µg per lane.

    goat anti-mouse HRP conjugated at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50&80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This image is courtesy of Britta Eickholt, Univ London Kings Coll, United Kingdom

References for Anti-Cre recombinase antibody [7.23] (ab24607)

This product has been referenced in:
  • Wu Y  et al. Ga12 is required for renal cystogenesis induced by Pkd1 inactivation. J Cell Sci 129:3675-3684 (2016). Read more (PubMed: 27505895) »
  • Ding J & Delpire E Deletion of KCC3 in parvalbumin neurons leads to locomotor deficit in a conditional mouse model of peripheral neuropathy associated with agenesis of the corpus callosum. Behav Brain Res 274:128-36 (2014). Mouse . Read more (PubMed: 25116249) »

See all 9 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry free floating
Rat Tissue sections (Brain tissue (Inferior colliculus and Medial Genic)
Brain tissue (Inferior colliculus and Medial Genic

Abcam user community

Verified customer

Submitted Jun 14 2017

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (mouse embryonic spinal cord (cryosections))
Yes - 0.1%Triton X-100
mouse embryonic spinal cord (cryosections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Dr. Frederic Clotman

Verified customer

Submitted Nov 19 2015

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Please check the following;

It is also used in other publications but we are unable to confirm the applications due to non availability of...

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Ante todo quisiera desearle un muy Feliz Año.

Muchas gracias por tomarte la molestia de rellenar el cuestionario y mandar más imágenes.

Hay alguna sugerencia que posiblemente mejore la tinción obtenida. M...

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Perhaps these are the antibodies customer is looking for;

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Ich möchte Ihnen hiermit nochmals viel Erfolg wünschen und drücke die Daumen, dass dieser Antikörper funktioniert.

Wenn Sie Ihre Versuche abgeschlossen haben würden wir uns über einen Abreview freuen. Die gesammel...

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Vielen Dank für diese zusätzliche Information.

Leider haben wir keinen Antikörper den ich für die Detektion Ihres rekombinanten Proteins garantieren kann. Ich habe drei Antikörper gefunden die vielleicht für Sie i...

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Thank you for contacting us.

We have 2 antibodies in our catalogwhich have beentested in IHC-Fr: ab24607, ab24580 (biotinylated).

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask...

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I am happy to offer a replacement from another lot or a credit for this antibody. Please let me know which you would prefer.

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