Anti-Cre recombinase antibody [DF6] (ab101071)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
  • Concentration information loading...
  • PurityConcentrated Culture Supernatant
  • ClonalityMonoclonal
  • Clone numberDF6
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Target

  • RelevanceCre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • Database links
    • Alternative names
      • Cre antibody
      • Cyclization recombinase antibody
      • Recombinase cre antibody

    Anti-Cre recombinase antibody [DF6] images

    • All lanes : Anti-Cre recombinase antibody [DF6] (ab101071) at 1 µg/ml

      Lane 1 : Cre Recombinase recombinant protein at 1 µg
      Lane 2 : Cre Recombinase recombinant protein at 2 µg

      Secondary
      Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 38 kDa
      Observed band size : 38 kDa


      Exposure time : 20 minutes

    References for Anti-Cre recombinase antibody [DF6] (ab101071)

    ab101071 has not yet been referenced specifically in any publications.

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    As we discussed, please let me know about the results after using the anti-mouse IgM secondary antibody. If there are still no bands on the blot, I wi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"