Anti-Cre recombinase antibody [DF6] (ab101071)

Overview

  • Product name
    Anti-Cre recombinase antibody [DF6]
    See all Cre recombinase primary antibodies
  • Description
    Mouse monoclonal [DF6] to Cre recombinase
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Bacteriophage P1
  • Immunogen

    Synthetic peptide Cre recombinase.

  • Positive control
    • This antibody gave a positive signal against a Cre Recombinase recombinant protein.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
  • Concentration information loading...
  • Purity
    Concentrated Culture Supernatant
  • Clonality
    Monoclonal
  • Clone number
    DF6
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Target

  • Relevance
    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • Database links
    • Alternative names
      • Cre antibody
      • Cyclization recombinase antibody
      • Recombinase cre antibody

    Images

    • All lanes : Anti-Cre recombinase antibody [DF6] (ab101071) at 1 µg/ml

      Lane 1 : Cre Recombinase recombinant protein at 1 µg
      Lane 2 : Cre Recombinase recombinant protein at 2 µg

      Secondary
      Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 38 kDa
      Observed band size : 38 kDa


      Exposure time : 20 minutes

    References

    ab101071 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your call today and for letting us know about the trouble with this antibody.

    As we discussed, please let me know about the results after using the anti-mouse IgM secondary antibody. If there are still no bands on the blot, I wi...

    Read More

    Thank you for contacting us.

    Unfortunately, because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products.

    However, we are happy to reassure our customers that all of our products are c...

    Read More

    I am very pleased to hear you would like to accept our offer and test ab101071 in IHC-FoFr.   This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-FoFr and include this c...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up