• Product name
    Creatine Assay Kit (Colorimetric/Fluorometric)
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • Assay type
  • Sensitivity
    > 0.001 mM
  • Range
    0.001 mM - 10 mM
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Product overview

    Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339) provides an accurate, convenient measure of creatine in a variety of biological samples. In the assay, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (?max = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001–10 mM Creatine.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development.

  • Tested applications
    Suitable for: Functional Studiesmore details


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Creatinase (Lyophilized) Blue 1 vial
    Creatine Assay Buffer WM, 1 x 25ml
    Creatine Enzyme Mix (Lyophilized) Green 1 vial
    Creatine Probe (in DMSO) Red 1 x 200µl
    Creatine Standard (10 µmol) (Lyophilized) Yellow 1 vial
  • Research areas
  • Relevance
    Creatine is an endogenous amino acid that occurs in vertebrate tissues and in urine. In muscle tissue, creatine is generally found as its phosphorylated form phosphocreatine. Creatine functions as part of the cell's energy shuttle: the high energy phosphate group of ATP is transferred to creatinine to form phosphocreatinine in the following reaction: Cr + ATP ? P-Cr + ADP. This reaction is reversibly catalized by creatine kinase. In the human body, creatine is synthesized mainly in the liver from arginine, glycine and methionine. Creatine is excreted as creatinine in the urine.

Associated products


Our Abpromise guarantee covers the use of ab65339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.


  • Creatine measured in cell lysates showing quantity (nmol) per 106 cells

  • Creatine measured in biologicals showing concentration (micromolar)



This product has been referenced in:
  • Wan ES  et al. Metabolomic profiling in a Hedgehog Interacting Protein (Hhip) murine model of chronic obstructive pulmonary disease. Sci Rep 7:2504 (2017). Read more (PubMed: 28566717) »

See 1 Publication for this product

Customer reviews and Q&As

It is correct that this kit can detect phosphorylated and non-phosphorylated creatine.

Sorry for any confusion here.
In the assay, creatine is enzymatically converted to sarcosine which is catalyzed by creatinase. This enzyme works at the first step of the detection reaction in the kit. This conversion event is not dependent on phos...

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Thank you for your enquiry and interest in our products.

Generally, most plate readers have flexibility in their bandwidth of detection. This is generally in terms of ±10, ±20 and so on. If your machine has the flexibility of at...

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Perdona por la espera.

He vuelto a comentar el caso con mis compañeros. Me temo que no tienen ninguna sugerencia que hacer respecto a un control positivo de Creatina para usar con el kit. Podríais utilizar Creatina pura en disti...

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Thank you for contacting us.

I have contacted the lab on your behalf. This product will work very well with mouse samples.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ad...

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Thank you for your phone call.

I can confirm that this kit will not be able to distinguish between phospho-creatine and creatine molecules. The enzyme used in this kit Creatine Amidinohydrolase which converts creatine into Sarcosine.


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Thank you for contacting us.
I have to confirm that we have not specifically tested with this sample type (tissue) and it is therefore not covered by our guarantee.
This kit should be compatible with all mammalian samples and therefore should...

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Thank you for contacting us.

The lab let me know the following:

The assay buffer composition is proprietary information. However, we recommend to use the buffer which comes with each kit, instead of substituting it with another kit...

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Thank you for your inquiry.

The ab65339 kit measures creatine, while the ab65340 kit measures creatinine.



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Thank you for contacting us.

This product has an expiration date of 6 months from date of arrival if stored at the recommended stated on the datasheet (-20C).

I hope this information is helpful to you. Please do not hesitate to cont...

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