Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)

Overview

  • Product name
    Creatine Kinase Activity Assay Kit (Colorimetric)
  • Sample type
    Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
  • Assay type
    Enzyme activity
  • Sensitivity
    < 1 mU/well
  • Range
    2 nmol/well - 10 nmol/well
  • Assay time
    1h 0m
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Mammal
  • Product overview

    Creatine Kinase Activity Assay kit (Colorimetric) (ab155901) is a kit where creatine kinase (CK) converts creatine into phosphocreatine and ADP. The generated phoshocreatine and ADP reacts with CK Enzyme Mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at ?= 450 nm. The CK Activity Assay is high-throughput adaptable, simple and sensitive. This assay kit can detect CK activity less than 1 mU.

  • Notes

    Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine. CK is widely expressed in various tissues and cell types, with highest activity in striated muscles, heart tissue and brain. CK consists of two subunits: M (muscle) and B (brain), and has three isoenzymes: CK-MM (skeleton muscle), CK-MB (cardiac muscle), and CK-BB (brain). Increased CK level is associated with many diseases such as myocardial infarction, muscular dystrophy, pulmonary infarction and brain tumors. Accurate measurement of CK is crucial for early diagnosis, prediction and therapeutic strategy.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    ATP Orange 1 vial
    CK Assay Buffer WM 1 x 25ml
    CK Developer Red 1 vial
    CK Enzyme Mix Green 1 vial
    CK Positive Control Purple 1 vial
    CK Substrate Blue 1 x 1ml
    NADH Standard Yellow 1 vial
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Creatine Kinase Activity measured in various samples.  

    Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.

  • Typical NADH standard calibration curve using colorimetric reading.

  • Creatine kinase activity tested in 5 µl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.

Protocols

References

This product has been referenced in:
  • Luptak I  et al. Decreased ATP production and myocardial contractile reserve in metabolic heart disease. J Mol Cell Cardiol 116:106-114 (2018). Read more (PubMed: 29409987) »
  • McRae N  et al. Glucocorticoids Improve Myogenic Differentiation In Vitro by Suppressing the Synthesis of Versican, a Transitional Matrix Protein Overexpressed in Dystrophic Skeletal Muscles. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 29211034) »

See all 4 Publications for this product

Customer reviews and Q&As

Creatine Kinase Activity Assay Kit (Colorimetric)

Excellent Excellent 5/5 (Ease of Use)
Abreviews
HIGHER QUALITY ANTIBODY
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Submitted Jan 24 2018

Abreviews
I used phenol red free DMEM media with 1% ITSS and 1% antibiotic/antimycotic. Dosed media was incubated with sheep cardiomyocytes for 48 hours and collected. Media was diluted 1:2 with molecular grade water and added to the kits reaction mix. Absorption readings were taken every 2 minutes for 40 minutes.


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Verified customer

Submitted Sep 08 2017

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The sample volume window is 1-50 ul. How much blood is used depends on the CK activity in the sample. When we used human serum (see data fig 1b on our datasheet), 5ul worked well.


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Thank you for your enquiry.
I can confirm that when using the ab155901 Creatine Kinase Activity Assay Kit there is no special sample preparation required for plasma samples.
The sample volume used per well will need to be optimized to get v...

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Creatine kinase in cells is either cytosolic or mitochondrial. The enzyme is not released into the medium under normal conditions. SO we would expect to see very low or no activity in ...

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I have contacted the lab and you do not need to add protease of phosphatase inhibitors for our kits unless otherwise stated. This is because, many of these inhibitors could chelate ions or adversely affect the enzymes in the assays.

Unfortunat...

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We recommend collecting the skeletal muscle samples and quick freeze in liquid nitrogen and store at -80C until they are ready to run all the samples together.

The tissue samples should be homogenized when they are ready to do the ex...

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The positive control activity may vary from batch to batch. It is included in the assay to confirm that the assay reagents are performing as expected. If the OD had been 0, then that would be a problem. An OD higher than the highest standard is not. To...

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The kit is guaranteed to be used with Rat samples. It is tested with rat heart lysates and we predict it will work with plasma samples as well.

This kit will detect total creatine kinase, including U-type. Unfortunately we do not have any specific data produced by the kit with brain homogenates.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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