The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family. Contains 1 phosphagen kinase C-terminal domain. Contains 1 phosphagen kinase N-terminal domain.
Western blot - Anti-Creatine kinase B type antibody [3A6] (ab71944)
Predicted band size : 43 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Creatine kinase B type knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lane 4: SHSY5Y cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab71944 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab71944 was shown to specifically react with Creatine kinase B type when Creatine kinase B type knockout samples were used. Wild-type and Creatine kinase B type knockout samples were subjected to SDS-PAGE. ab71944 and ab181602 (loading control to GAPDH) were diluted at 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goGoat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Creatine Kinase BB antibody [3A6] (ab71944)
All lanes : Anti-Creatine kinase B type antibody [3A6] (ab71944) at 1/2000 dilution
Lane 1 : Recombinant CK-MM protein isolated from E. coli Lane 2 : Recombinant CK-BB protein isolated from E. coli Lane 3 : Recombinant CK-MB protein isolated from E. coli Lane 4 : Human CK-MB protein