Anti-Creatine kinase B type antibody [3A6] (ab71944)


  • Product nameAnti-Creatine kinase B type antibody [3A6]
    See all Creatine kinase B type primary antibodies
  • Description
    Mouse monoclonal [3A6] to Creatine kinase B type
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Creatine kinase B type.

  • Positive control
    • Human Creatine Kinase MB protein. Recombinant Creatine Kinase BB protein. Recombinant Creatine Kinase MB protein.



Our Abpromise guarantee covers the use of ab71944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).


  • FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine kinase brain type antibody
    • Creatine phosphokinase BB antibody
    • Epididymis luminal protein 211 antibody
    • Epididymis secretory protein Li 29 antibody
    • HEL 211 antibody
    • HEL S 29 antibody
    • KCRB_HUMAN antibody
    see all

Anti-Creatine kinase B type antibody [3A6] images

  • Predicted band size : 43 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Creatine kinase B type knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: SHSY5Y cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab71944 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab71944 was shown to specifically react with Creatine kinase B type when Creatine kinase B type knockout samples were used. Wild-type and Creatine kinase B type knockout samples were subjected to SDS-PAGE. ab71944 and ab181602 (loading control to GAPDH) were diluted at 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goGoat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Creatine kinase B type antibody [3A6] (ab71944) at 1/2000 dilution

    Lane 1 : Recombinant CK-MM protein isolated from E. coli
    Lane 2 : Recombinant CK-BB protein isolated from E. coli
    Lane 3 : Recombinant CK-MB protein isolated from E. coli
    Lane 4 : Human CK-MB protein

    Predicted band size : 43 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

References for Anti-Creatine kinase B type antibody [3A6] (ab71944)

ab71944 has not yet been referenced specifically in any publications.

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