Anti-Creatine kinase B type antibody (ab38211)

Overview

  • Product nameAnti-Creatine kinase B type antibody
    See all Creatine kinase B type primary antibodies
  • Description
    Rabbit polyclonal to Creatine kinase B type
  • SpecificityThis antibody is specific for Creatine kinase BB.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected within aa 200~300 of human Creatine Kinase BB.

  • Positive control
    • Y79 cell lysate and mouse colon tissue lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab38211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine phosphokinase BB antibody
    • KCRB_HUMAN antibody
    see all

Anti-Creatine kinase B type antibody images

  • All lanes : Anti-Creatine kinase B type antibody (ab38211) at 1/100 dilution

    Lane 1 : Y79 cell lysate
    Lane 2 : Mouse colon tissue lysate


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • Anti-Creatine kinase B type antibody (ab38211) at 1/250 dilution + Tissue lysate prepared from human brain tissue at 10 µg

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/7500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa


    Exposure time : 1 minute

    This image is a courtesy of Anonymous Abreview

    See Abreview

References for Anti-Creatine kinase B type antibody (ab38211)

This product has been referenced in:
  • Mochel F  et al. Early alterations of brain cellular energy homeostasis in huntington disease models. J Biol Chem 287:1361-70 (2012). WB ; Mouse . Read more (PubMed: 22123819) »
  • Zhang SF  et al. Impaired brain creatine kinase activity in Huntington's disease. Neurodegener Dis 8:194-201 (2011). Mouse . Read more (PubMed: 21124007) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us.

I have checked the following alignments with the following homology results:
CK BB: human-mouse: 97%
CK MM: human-mouse: 97%
CK MB: human-mouse: 97% - there is no separate sequence for MB as the pr...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Brain)
Loading amount 10 µg
Specification Brain
Treatment homogenisation in guanidine thiocyanate with subsequent ultracentrifugation over caesium chloride and dialysis
Gel Running Conditions Reduced Denaturing (4-20%, 1mm)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (brain)
Loading amount 5 µg
Specification brain
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"