The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family. Contains 1 phosphagen kinase C-terminal domain. Contains 1 phosphagen kinase N-terminal domain.
Western blot - Creatine Kinase BB antibody (ab38212)
All lanes : Anti-Creatine kinase B type antibody (ab38212) at 1/100 dilution
Lane 1 : Mouse colon tissue lysate Lane 2 : Y79 cell lysate
Predicted band size : 43 kDa Observed band size : 43 kDa
References for Anti-Creatine kinase B type antibody (ab38212)
This product has been referenced in:
Goonetilleke UR et al. Death is associated with complement C3 depletion in cerebrospinal fluid of patients with pneumococcal meningitis. MBio3: (2012).
Read more (PubMed: 22415003) »
Glen A et al. iTRAQ-Facilitated Proteomic Analysis of Human Prostate Cancer Cells Identifies Proteins Associated with Progression. J Proteome Res7:897-907 (2008).
Read more (PubMed: 18232632) »