Anti-Creatine kinase B type antibody (ab97546)

Overview

  • Product nameAnti-Creatine kinase B type antibody
    See all Creatine kinase B type primary antibodies
  • Description
    Rabbit polyclonal to Creatine kinase B type
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Zebrafish
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 165-357 (LDGDLAGRYY ALKSMTEAEQ QQLIDDHFLF DKPVSPLLLA SGMARDWPDA RGIWHNDNKT FLVWVNEEDH LRVISMQKGG NMKEVFTRFC TGLTQIETLF KSKDYEFMWN PHLGYILTCP SNLGTGLRAG VHIKLPNLGK HEKFSEVLKR LRLQKRGTGG VDTAAVGGVF DVSNADRLGF SEVELVQMVV DGV) of Creatine Kinase BB (NP_001814).

  • Positive control
    • WB: H1299 and HeLa S3 cell lysates ICC/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab97546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100 - 1/200.

Target

Anti-Creatine kinase B type antibody images

  • All lanes : Anti-Creatine kinase B type antibody (ab97546) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 43 kDa
  • Immunofluorescence analysis of Creatine Kinase BB in paraformaldehyde fixed HeLa, using ab97546 at 1/100 dilution.
    Lower image is merged with DNA probe.

References for Anti-Creatine kinase B type antibody (ab97546)

ab97546 has not yet been referenced specifically in any publications.

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Fixation

Fix the samples either in ice-cold methanol, acetone (1-10 min) or in 3-4% paraformaldehyde in PBS pH 7.4 for 15 min at room temperatu...

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