Overview

  • Product name
    Anti-Creatine kinase B type antibody
    See all Creatine kinase B type primary antibodies
  • Description
    Rabbit polyclonal to Creatine kinase B type
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Zebrafish
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 165-357 (LDGDLAGRYY ALKSMTEAEQ QQLIDDHFLF DKPVSPLLLA SGMARDWPDA RGIWHNDNKT FLVWVNEEDH LRVISMQKGG NMKEVFTRFC TGLTQIETLF KSKDYEFMWN PHLGYILTCP SNLGTGLRAG VHIKLPNLGK HEKFSEVLKR LRLQKRGTGG VDTAAVGGVF DVSNADRLGF SEVELVQMVV DGV) of Creatine Kinase BB (NP_001814).

  • Positive control
    • WB: H1299 and HeLa S3 cell lysates ICC/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab97546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities
    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine kinase brain type antibody
    • Creatine phosphokinase BB antibody
    • Epididymis luminal protein 211 antibody
    • Epididymis secretory protein Li 29 antibody
    • HEL 211 antibody
    • HEL S 29 antibody
    • KCRB_HUMAN antibody
    see all

Anti-Creatine kinase B type antibody images

  • All lanes : Anti-Creatine kinase B type antibody (ab97546) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 43 kDa
  • Immunofluorescence analysis of Creatine Kinase BB in paraformaldehyde fixed HeLa, using ab97546 at 1/100 dilution.
    Lower image is merged with DNA probe.

References for Anti-Creatine kinase B type antibody (ab97546)

This product has been referenced in:
  • Mahendrasingam S  et al. Subcellular distribution and relative expression of fibrocyte markers in the CD/1 mouse cochlea assessed by semiquantitative immunogold electron microscopy. J Histochem Cytochem 59:984-1000 (2011). Read more (PubMed: 22043022) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the or...

Read More

Thank you so much for your call today and for letting us know about the trouble with the staining.

As we discussed, I'm sending a free of charge DAB avidin-biotin complex staining kit, ab64261, which should arrive on Monday on the order ***. ...

Read More

Thank you for contacting us. I would recommend the following protocol for your purposes:

Fixation

Fix the samples either in ice-cold methanol, acetone (1-10 min) or in 3-4% paraformaldehyde in PBS pH 7.4 for 15 min at room temperatu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up