Anti-Creatine kinase B type antibody [CK-BYK/21E10] (ab125114)

Overview

  • Product name
    Anti-Creatine kinase B type antibody [CK-BYK/21E10]
    See all Creatine kinase B type primary antibodies
  • Description
    Mouse monoclonal [CK-BYK/21E10] to Creatine kinase B type
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    SNSHNALKLRFPAEDEF

    , corresponding to N terminal amino acids 4-20 of Human Creatine kinase B type.

  • Positive control
    • Human Purkinje cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab125114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50. Fixed 2 hr in 2% paraformaldehyde and 0.1 % glutaraldehyde in 0.1 M PB.
ICC/IF Use at an assay dependent concentration.
WB 1/50. Detects a band of approximately 43-46 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. 0.5 % pepsin in 0.01 N HCl for 15 min at 37 °C.

Target

  • Function
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities
    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine kinase brain type antibody
    • Creatine phosphokinase BB antibody
    • Epididymis luminal protein 211 antibody
    • Epididymis secretory protein Li 29 antibody
    • HEL 211 antibody
    • HEL S 29 antibody
    • KCRB_HUMAN antibody
    see all

Images



  • Predicted band size : 43 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Creatine kinase B knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: SH-SY5Y cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab125114 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab125114 was shown to recognize Creatine kinase B when Creatine kinase B knockout samples were used, along with additional cross-reactive bands. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab125114 and ab181602 (loading control to GAPDH) were diluted 1/50 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW)preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

References

This product has been referenced in:
  • Glover LE  et al. Control of creatine metabolism by HIF is an endogenous mechanism of barrier regulation in colitis. Proc Natl Acad Sci U S A 110:19820-5 (2013). Read more (PubMed: 24248342) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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