Anti-Creatine kinase B type antibody [CK-BYK/21E10] (Biotin) (ab125123)

Overview

  • Product name
    Anti-Creatine kinase B type antibody [CK-BYK/21E10] (Biotin)
    See all Creatine kinase B type primary antibodies
  • Description
    Mouse monoclonal [CK-BYK/21E10] to Creatine kinase B type (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    SNSHNALKLRFPAEDEF

    , corresponding to N terminal amino acids 4-20 of Human Creatine kinase B type.

  • Positive control
    • Human Purkinje cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab125123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Detects a band of approximately 43-46 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. antigen retrieval: 0.5 % pepsin in 0.01 N HCl for 15 min at 37 °C.
IHC-Fr 1/50. Fixed 2 hr in 2% paraformaldehyde and 0.1 % glutaraldehyde.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities
    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine kinase brain type antibody
    • Creatine phosphokinase BB antibody
    • Epididymis luminal protein 211 antibody
    • Epididymis secretory protein Li 29 antibody
    • HEL 211 antibody
    • HEL S 29 antibody
    • KCRB_HUMAN antibody
    see all

References

ab125123 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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