The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50. Detects a band of approximately 43-46 kDa (predicted molecular weight: 43 kDa).
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. antigen retrieval: 0.5 % pepsin in 0.01 N HCl for 15 min at 37 °C.
1/50. Fixed 2 hr in 2% paraformaldehyde and 0.1 % glutaraldehyde.
Use at an assay dependent dilution.
FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family. Contains 1 phosphagen kinase C-terminal domain. Contains 1 phosphagen kinase N-terminal domain.