Validated using a knockout cell line
Recombinant
RabMAb

Anti-Creatine kinase B type antibody [EPR3927] (ab92452)

Overview

  • Product name
    Anti-Creatine kinase B type antibody [EPR3927]
    See all Creatine kinase B type primary antibodies
  • Description
    Rabbit monoclonal [EPR3927] to Creatine kinase B type
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human Creatine Kinase B type.

  • Positive control
    • SHSY-5Y, HeLa, Y-79 and SW840 cell lysate. Human glioma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/500.

Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining.

Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
    • Sequence similarities
      Belongs to the ATP:guanido phosphotransferase family.
      Contains 1 phosphagen kinase C-terminal domain.
      Contains 1 phosphagen kinase N-terminal domain.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • B CK antibody
      • B-CK antibody
      • BB-CK antibody
      • BCK antibody
      • Brain creatine kinase antibody
      • Ckb antibody
      • CKBB antibody
      • Creatine kinase B antibody
      • Creatine kinase B chain antibody
      • Creatine kinase B type antibody
      • Creatine kinase B-type antibody
      • Creatine Kinase BB Isoenzyme antibody
      • Creatine kinase brain antibody
      • Creatine kinase brain type antibody
      • Creatine phosphokinase BB antibody
      • Epididymis luminal protein 211 antibody
      • Epididymis secretory protein Li 29 antibody
      • HEL 211 antibody
      • HEL S 29 antibody
      • KCRB_HUMAN antibody
      see all

    Images



    • Predicted band size : 43 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Creatine kinase B knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: SH-SY5Y cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab92452 observed at 45 kDa. Red - loading control, ab18058, observed at 2X kDa.

      ab92452 was shown to specifically react with Creatine kinase B when ProteinX knockout samples were used. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab92452 and ab18058 (loading control to vinculin) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Creatine kinase B type antibody [EPR3927] (ab92452) at 1/10000 dilution

      Lane 1 : SHSY-5Y cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Y-79 cell lysate
      Lane 4 : SW840 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 43 kDa
    • Paraffin embedded Human glioma tissue labelled with ab92452 at 1/100 dilution.
    • Immunofluorescence analysis of HeLa cells labelled with ab92452 at 1/100 dilution.
    • Overlay histogram showing HeLa cells stained with ab92452 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92452, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References

    ab92452 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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