Anti-Creatine kinase B type antibody [ZN-1] (ab170175)

Overview

  • Product name
    Anti-Creatine kinase B type antibody [ZN-1]
    See all Creatine kinase B type primary antibodies
  • Description
    Mouse monoclonal [ZN-1] to Creatine kinase B type
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Zebrafish
    Does not react with: Mouse, Rat, Cow, Human
  • Immunogen

    Tissue, cells or virus corresponding to Zebrafish Creatine kinase B type. Zebrafish Organism
    Database link: Q8AY63

  • Positive control
    • Brain Homogenate lysates.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab170175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.

Primary overnight incubation.

IP Use at an assay dependent concentration.

Target

  • Function
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities
    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine kinase brain type antibody
    • Creatine phosphokinase BB antibody
    • Epididymis luminal protein 211 antibody
    • Epididymis secretory protein Li 29 antibody
    • HEL 211 antibody
    • HEL S 29 antibody
    • KCRB_HUMAN antibody
    see all

Images

  • All lanes : Anti-Creatine kinase B type antibody [ZN-1] (ab170175) at 2 µg/ml

    Lane 1 : ZBH (Zebrafish Brain Homogenate) Lysate
    Lane 2 : ZSkMH (Zebrafish Skeletal Muscle Homogenate) Lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG –HRP at 1/1000 dilution

    Predicted band size: 43 kDa

  • Immunoprecipitation for Creatine Kinase B type (ZN-1) antibody.

    ab170175 conjugated to Protein-G-Beads used at 20 µg antibody per 10 µL of beads. Immunoprecipitated from 1 mg Zebrafish Brain Homogenate Lysate. 25 µL eluate was loaded onto the gel.

    Stained with Colloidal Coomassie Blue.

References

ab170175 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170175.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up