Overview

  • Product nameAnti-Creatine Kinase MB antibody [CK1]
    See all Creatine Kinase MB primary antibodies
  • Description
    Mouse monoclonal [CK1] to Creatine Kinase MB
  • SpecificityCK1 recognises human creatine kinase MB isoenzyme. Its affinity for the creatine kinase BB isoenzyme is greater than its affinity for creatine kinase MB isoenzyme. hCK-MB - 100% hCK-BB - 182% hCK-MM - 7.3% Affinity constant: 2x109 l/mol human CK-MB.
  • Tested applicationsSuitable for: ELISA, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

Target

  • RelevanceCreatine Kinase MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Creatine Kinase MB reversibly catalyses the transfer of phosphate between ATP and various phosphogens. The creatine kinase isoenzymes play a central role in energy transduction in tissues with large fluctuating energy demands such as skeletal muscle, heart, brain and spermatozoa.
  • Cellular localizationCytoplasmic and Mitochondrial
  • Database links
  • Alternative names
    • B-CK antibody
    • brain creatine kinase antibody
    • CKBB antibody
    • CKMB antibody
    • CKMM antibody
    • creatine kinase B antibody
    • creatine kinase brain antibody
    • creatine kinase M antibody
    • Creatine kinase MB antibody
    • creatine kinase muscle antibody
    • M-CK antibody
    • muscle creatine kinase antibody
    see all

References for Anti-Creatine Kinase MB antibody [CK1] (ab404)

This product has been referenced in:
  • Garay F  et al. Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples. Anal Bioanal Chem 397:1873-81 (2010). Read more (PubMed: 20449577) »
  • Crawford RM  et al. Creatine kinase is physically associated with the cardiac ATP-sensitive K+ channel in vivo. FASEB J 16:102-4 (2002). Read more (PubMed: 11729098) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. I can confirm the pI range for ab404 Creatine Kinase MB antibody [CK1] is 6.9 to 8.3. Antibodies have ranges of pI rather than points. I hope this information is helpful to you. Should you have any further questions,...

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All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and...

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This antibody has not been tested in immunoprecipitation, so we can't guarantee that it will work.

Dear Gary Please see my answer to your question on ab8364. This is essentially repeated below. This antibody hasn't been tested in Westerns. It is a monoclonal and it works in ELISA and Radioimmunoassays. It is possible that it works in Western...

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This antibody has not been tested in Western blotting applications, but a good starting dilution for both ELISA and Western would be 1/1000.

This antibody has not been tested for cross-reactivity against mouse. However as it was raised in mouse, cross-reactivity against the mouse antigen is unlikely.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"