The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family. Contains 1 phosphagen kinase C-terminal domain. Contains 1 phosphagen kinase N-terminal domain.
Western blot - Creatine Kinase MM antibody [2C5] (ab72004)
All lanes : Anti-Creatine Kinase MM antibody [2C5] (ab72004) at 1/1000 dilution
Lane 1 : Recombinant human Creatine Kinase MM protein isolated from E. coli Lane 2 : Recombinant human Creatine Kinase BB protein isolated from E. coli Lane 3 : Recombinant human Creatine Kinase MB protein isolated from E. coli Lane 4 : Human Creatine Kinase MB protein