Overview

  • Product name
    Anti-Creatine Kinase MM antibody
    See all Creatine Kinase MM primary antibodies
  • Description
    Mouse monoclonal to Creatine Kinase MM
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: TCPSNLGTGL RGGVHVKLAH LSKHPKFEEI LTRLRLQKRG TGGVDTAAVG SVFDVSNADR LGSSEVEQVQ LVVDGVKLMV EMEKKLEKGQ SIDDMIPAQK , corresponding to amino acids 282-382 of Human Creatine Kinase MM

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab189438 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab54637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities
    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CKM antibody
    • CKMM antibody
    • Creatine kinase M antibody
    • Creatine kinase M chain antibody
    • Creatine kinase M type antibody
    • Creatine kinase M-type antibody
    • Creatine kinase muscle antibody
    • Creatine kinase, muscle type antibody
    • KCRM_HUMAN antibody
    • M-CK antibody
    • MCK antibody
    • Muscle creatine kinase antibody
    see all

Images



  • Predicted band size : 43 kDa
    Creatine Kinase MM antibody (ab54637) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • ab54637 at 10 µg/ml staining Creatine Kinase MM in human Hela cells by Immunocytochemistry / Immunofluorescence.

References

This product has been referenced in:
  • Zhang GJ  et al. An integrated chip for rapid, sensitive, and multiplexed detection of cardiac biomarkers from fingerprick blood. Biosens Bioelectron 28:459-63 (2011). Read more (PubMed: 21807497) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Rafael Montes Nieto

Verified customer

Submitted Oct 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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