Overview

  • Product nameAnti-Creatine Kinase MM antibody
    See all Creatine Kinase MM primary antibodies
  • Description
    Mouse monoclonal to Creatine Kinase MM
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: TCPSNLGTGL RGGVHVKLAH LSKHPKFEEI LTRLRLQKRG TGGVDTAAVG SVFDVSNADR LGSSEVEQVQ LVVDGVKLMV EMEKKLEKGQ SIDDMIPAQK , corresponding to amino acids 282-382 of Human Creatine Kinase MM

Properties

Applications

Our Abpromise guarantee covers the use of ab54637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CKM antibody
    • CKMM antibody
    • Creatine kinase M antibody
    • Creatine kinase M chain antibody
    • Creatine kinase M type antibody
    • Creatine kinase M-type antibody
    • Creatine kinase muscle antibody
    • Creatine kinase, muscle type antibody
    • KCRM_HUMAN antibody
    • M-CK antibody
    • MCK antibody
    • Muscle creatine kinase antibody
    see all

Anti-Creatine Kinase MM antibody images



  • Predicted band size : 43 kDa
    Creatine Kinase MM antibody (ab54637) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • ab54637 at 10 µg/ml staining Creatine Kinase MM in human Hela cells by Immunocytochemistry / Immunofluorescence.

References for Anti-Creatine Kinase MM antibody (ab54637)

This product has been referenced in:
  • Zhang GJ  et al. An integrated chip for rapid, sensitive, and multiplexed detection of cardiac biomarkers from fingerprick blood. Biosens Bioelectron 28:459-63 (2011). Read more (PubMed: 21807497) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Tissue lysate - whole (skeletal muscle)
Specification skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Rafael Montes Nieto

Verified customer

Submitted Oct 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"