Anti-Creatine Kinase MM antibody [ZM-2] (ab170174)

Overview

  • Product name
    Anti-Creatine Kinase MM antibody [ZM-2]
    See all Creatine Kinase MM primary antibodies
  • Description
    Mouse monoclonal [ZM-2] to Creatine Kinase MM
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Zebrafish
    Does not react with: Mouse, Rat, Cow, Human
  • Immunogen

    Tissue, cells or virus corresponding to Zebrafish Creatine Kinase MM. Zebrafish Organism.
    Database link: Q9PV91

  • Positive control
    • Zebrafish Skeletal Muscle Homogenate.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab170174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
IP Use at an assay dependent concentration.

Target

  • Function
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities
    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Alternative names
    • CKM antibody
    • CKMM antibody
    • Creatine kinase M antibody
    • Creatine kinase M chain antibody
    • Creatine kinase M type antibody
    • Creatine kinase M-type antibody
    • Creatine kinase muscle antibody
    • Creatine kinase, muscle type antibody
    • KCRM_HUMAN antibody
    • M-CK antibody
    • MCK antibody
    • Muscle creatine kinase antibody
    see all

Images

  • All lanes : Anti-Creatine Kinase MM antibody [ZM-2] (ab170174) at 1 µg/ml

    Lane 1 : ZBH (Zebrafish Brain Homogenate) Lysate
    Lane 2 : ZSkMH (Zebrafish Skeletal Muscle Homogenate) Lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG – HRP at 1/2000 dilution

    Predicted band size: 43 kDa

  • Immunoprecipitation for Creatine Kinase MM  (ZM-2) antibody.

    ab170174 conjugated to Protein-G-Beads used at 20 µg antibody per 10 µL of beads. Immunoprecipitated from 1 mg Zebrafish Skeletal Muscle Homogenate Lysate. 25µL eluate was loaded onto gel.

    Stained with Colloidal Coomassie Blue.

References

ab170174 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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