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Recombinant fragment: GVHIKLPLLS KDSRFPKILE NLRLQKRGTG GVDTAATGGV FDISNLDRLG KSEVELVQLV IDGVNYLIDC ERRLERGQDI RIPTPVIHTK H, corresponding to amino acids 327-418 of Human Creatine kinase MT (NP_066270) with a tag of 26 kDa.
Our Abpromise guarantee covers the use of ab76506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 46 kDa. A 72kDa faint band could be seen in WB. We are unsure of its identity.|
|ELISA||Use at an assay dependent concentration. Detection limit for recombinant tagged Creatine kinase MT is approximately 0.1ng/ml if used as a capture antibody.|
|IHC-P||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|