• Product nameCreatinine Assay Kit
    See all Creatinine kits
  • Detection methodColorimetric/Fluorometric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Product overview

    Creatinine Assay Kit (ab65340) provides an accurate, convenient measure of creatine concentration in biological fluids such as serum, urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm). Unlike picric acid assays, this kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.

    For deproteinization of samples:  10kda method works the beter for deproteinization when preparing samples as opposed to PCA method. It’s possible that the chemicals used negatively affects the enzyme components of the assay kit.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Creatinine is a breakdown product of creatine phosphate. Creatinine is produced and excreted at a constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function. Blood creatinine levels increase only in cases of significant (>75%) damage to nephrons. Creatinine clearance is frequently used as a means of standardizing excretion of other compounds such as isoprostanes.

  • Tested applicationsSuitable for: Functional Studiesmore details


  • RelevanceCreatinine, or creatine anhydride, is a breakdown product of creatine phosphate in muscle. The loss of water molecule from creatine results in the formation of creatinine. Creatinine is transferred to the kidneys by blood plasma, whereupon it is eliminated from the body by glomerular filtration and partial tubular excretion. Creatinine is usually produced and excreted at a fairly constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function.

Associated products


Our Abpromise guarantee covers the use of ab65340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Creatinine Assay Kit images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Creatinine measured in mouse and human serum plotted against RFU. Samples were diluted 5-10 fold.

  • Creatinine measured in mouse and human urine plotted against RFU. Samples were diluted 400-800 fold.

  • Creatinine levels in filtered human urine was measured in the presence or absence of creatininase (background signal subtracted.


References for Creatinine Assay Kit (ab65340)

This product has been referenced in:
  • Gai Z  et al. Farnesoid X Receptor Protects against Kidney Injury in Uninephrectomized Obese Mice. J Biol Chem 291:2397-411 (2016). Read more (PubMed: 26655953) »
  • Elsaid KA  et al. The impact of early intra-articular administration of interleukin-1 receptor antagonist on lubricin metabolism and cartilage degeneration in an anterior cruciate ligament transection model. Osteoarthritis Cartilage 23:114-21 (2015). Functional Studies . Read more (PubMed: 25219670) »

See all 15 Publications for this product

Product Wall

Creatinine in porcine urine samples

Excellent Excellent 5/5 (Ease of Use)
Tested the kit in porcine urine samples and after deproteinisation, the kit worked. We used the colourimetric assay as per protocol in the booklet.
We used the deproteinisation protocol with PCA and KOH as provided in the booklet that accompanies the kit.
Had to dilute the deproteinised samples 50x to bring samples into the expected detectable range.

Abcam user community

Verified customer

Submitted Jul 28 2015

I can confirm that frozen samples can be used for accurate results with this kit.

However, the success of the storage and quality of the sample will depend on multiple criteria. This would including the length of storage, the number of ro...

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Creatine is a non-protein amino acid and therefore exists in the same form in all animals.

This Creatinine Assay Kit (ab65340) is therefore species independent which means that it can be used with all and any species which have creatinine. Th...

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I have contacted the lab who have confirmed that they recommend Heparin over EDTA when preparing blood samples for use with this kit.

Please add background mix to standard and samples in separate wells? We recomend performing this in duplicate and then subtracting the mean from the original reading of standard and sample.

We recommend diluting the probe 10x and then using 0.4ul of the probe for the best results for the fluorometric assay. Typically this works the best. This assay is very sensitive and having too much probe will result in very high RFU values beyond the ...

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We have not tested this kit individually in higher than physiological amounts of the moieties you mentioned. In typical samples, to our knowledge sarcosine and creatine can interfere with the assay which is resolved by running appropriate controls. Sin...

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Gracias por contactarnos.
Este kit esta diseñado para mamíferos, y su límite de detección vendrá determinado por el valor más bajo de la lectura siempre que caiga dentro del rango linear de la recta estándar. Por ejemplo, el suero contiene entre 4...

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I have contacted the lab for additional advice.

My suggestions -as we discuss over the phone - would be:
1) check the creatine and sarcosine background
2) use serum instead of plasma
3) deproteinize the samples as recommended ...

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I can confirm that this kit can be used for measuring creatinine, not creatine kinase. (creatine kinase level is a marker for myocardial infarction).

However we are not specialized in this particular field and we cannot determine if...

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