Creatinine Assay Kit (Colorimetric) (ab204537)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Urine
Overview
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Product name
Creatinine Assay Kit (Colorimetric)
See all Creatinine kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 7.981 mg/dl 20 = 2.159% 7.452 mg/dl 20 = 3.203% 2.007 mg/dl 20 = 5.995% 7.326 mg/dl 20 = 3.729% Inter-assay Sample n Mean SD CV% 7.591 mg/dl 20 = 2.249% 7.232 mg/dl 20 = 2.052% 1.932 mg/dl 20 = 2.027% 7.085 mg/dl 20 = 1.433% -
Sample type
Urine -
Assay type
Quantitative -
Recovery
Sample specific recovery Sample type Average % Range Urine = 95 92% - 99% -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Creatinine Assay Kit (Colorimetric) (ab204537) is a complete kit for the quantitative determination of creatinine in urine, and is based upon the Jaffe reaction.
Sensitivity = 0.042 mg/dL.
General range = 0.31 - 20 mg/dL
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Notes
Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted nonenzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is <15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 2 x 96 tests Clear Microtiter Plates 2 units Creatinine Detection Reagent 1 x 20ml Creatinine Standard 1 vial Plate Sealer 2 units -
Research areas
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Relevance
Creatinine, or creatine anhydride, is a breakdown product of creatine phosphate in muscle. The loss of water molecule from creatine results in the formation of creatinine. Creatinine is transferred to the kidneys by blood plasma, whereupon it is eliminated from the body by glomerular filtration and partial tubular excretion. Creatinine is usually produced and excreted at a fairly constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function.
Images
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab204537 has been referenced in 8 publications.
- Widjaja AA et al. A Neutralizing IL-11 Antibody Improves Renal Function and Increases Lifespan in a Mouse Model of Alport Syndrome. J Am Soc Nephrol 33:718-730 (2022). PubMed: 35140116
- Fan LL et al. Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells. Exp Mol Med 54:653-661 (2022). PubMed: 35596061
- Gu W et al. Pterostilbene, a Resveratrol Derivative, Improves Ectopic Lipid Deposition in the Kidneys of Mice Induced by a High-Fat Diet. Kidney Blood Press Res 47:514-522 (2022). PubMed: 35717941
- ShamsEldeen AM et al. Combined Systemic Intake of K-ATP Opener (Nicorandil) and Mesenchymal Stem Cells Preconditioned With Nicorandil Alleviates Pancreatic Insufficiency in a Model of Bilateral Renal Ischemia/Reperfusion Injury. Front Physiol 13:934597 (2022). PubMed: 35812319
- Adrianowycz SE et al. Establishment of an RI for the urine ammonia-to-creatinine ratio in dogs. Vet Clin Pathol 50:597-602 (2021). PubMed: 34854107
- Ryu H et al. Urinary creatinine varies with microenvironment and sex in hibernating Greater Horseshoe bats (Rhinolophus ferrumequinum) in Korea. BMC Ecol Evol 21:77 (2021). PubMed: 33947328
- Heidenreich E et al. A Novel UPLC-MS/MS Method Identifies Organ-Specific Dipeptide Profiles. Int J Mol Sci 22:N/A (2021). PubMed: 34576148
- Pierzynowska K et al. Oral Treatment With an Engineered Uricase, ALLN-346, Reduces Hyperuricemia, and Uricosuria in Urate Oxidase-Deficient Mice. Front Med (Lausanne) 7:569215 (2020). PubMed: 33330529