The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 37 kDa.
Use at an assay dependent concentration.
1/400. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThis protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
Involvement in diseaseDefects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
Sequence similaritiesBelongs to the bZIP family. Contains 1 bZIP domain. Contains 1 KID (kinase-inducible) domain.
Post-translational modificationsStimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
cAMP responsive element binding protein 1 antibody
cAMP-responsive element-binding protein 1 antibody
Cyclic AMP-responsive element-binding protein 1 antibody
Transactivator protein antibody
Anti-CREB antibody [48H2] images
Western blot - Anti-CREB antibody [48H2] (ab49762)
Predicted band size : 37 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: CREB knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lane 4: A431 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab49762 observed at 44 kDa. Red - loading control, ab18058, observed at 124 kDa. ab49762 was shown to recognize CREB when CREB knockout samples were used, along with additional cross-reactive bands. Wild-type and CREB knockout samples were subjected to SDS-PAGE. ab49762 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Frozen sections) - Anti-CREB antibody [48H2] (ab49762)This image is courtesy of an anonymous Abreview.
ab49762 staining CREB in rat brain cortex and cerebellum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed using a zinc fixative and blocked with biotin for 16 minutes at 37°C. Samples were incubated with primary antibody (9 ug/mL) for 1 hour at 37°C. A biotinylated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
References for Anti-CREB antibody [48H2] (ab49762)
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