The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-Fo: 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 39 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThis protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
Involvement in diseaseDefects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
Sequence similaritiesBelongs to the bZIP family. Contains 1 bZIP domain. Contains 1 KID (kinase-inducible) domain.
Post-translational modificationsStimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
cAMP responsive element binding protein 1 antibody
cAMP-responsive element-binding protein 1 antibody
Cyclic AMP-responsive element-binding protein 1 antibody
Transactivator protein antibody
Anti-CREB antibody images
Western blot - CREB antibody (ab11924)
Predicted band size : 39 kDa
ab11924 at 1/1000 dilution staining Human CREB in A431 cells (nuclear extract) by Western blot (ECL).
Immunohistochemistry (PFA perfusion fixed frozen sections) - CREB antibody (ab11924)Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
CREB antibody (ab11924) at a dilution of 1/100 (2 X o/n incubation at RT; ABC amplification). Strong nuclear staining observed in spinal cord [A] 20x objective, [B] 40x objective. Staining performed in free floating IHC on 30µm saggital spinal cord sections. Find detailed information regarding the protocol in the Specific Protocol link.
Kammerer S et al. Critical evaluation of KCNJ3 gene product detection in human breast cancer: mRNA in situ hybridisation is superior to immunohistochemistry. J Clin Pathol69:1116-1121 (2016).
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