Overview

  • Product nameAnti-CREB antibody
    See all CREB primary antibodies
  • Description
    Rabbit polyclonal to CREB
  • Tested applicationsSuitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 6 - 23 of Human CREB.

  • Positive control
    • A-431 nuclear extract.
  • General notesKeep on ice when not in storage.

Properties

Applications

Our Abpromise guarantee covers the use of ab11924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
WB
  • Application notesIHC-Fo: 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 39 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
    • Involvement in diseaseDefects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
    • Sequence similaritiesBelongs to the bZIP family.
      Contains 1 bZIP domain.
      Contains 1 KID (kinase-inducible) domain.
    • Post-translational
      modifications
      Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Active transcription factor CREB antibody
      • cAMP response element binding protein 1 antibody
      • cAMP response element binding protein antibody
      • cAMP responsive element binding protein 1 antibody
      • cAMP-responsive element-binding protein 1 antibody
      • CREB antibody
      • CREB-1 antibody
      • CREB1 antibody
      • CREB1_HUMAN antibody
      • Cyclic AMP-responsive element-binding protein 1 antibody
      • MGC9284 antibody
      • OTTHUMP00000163864 antibody
      • OTTHUMP00000163865 antibody
      • OTTHUMP00000206660 antibody
      • OTTHUMP00000206662 antibody
      • OTTHUMP00000206667 antibody
      • Transactivator protein antibody
      see all

    Anti-CREB antibody images



    • Predicted band size : 39 kDa


      ab11924 at 1/1000 dilution staining Human CREB in A431 cells (nuclear extract) by Western blot (ECL).
    • CREB antibody (ab11924) at a dilution of 1/100 (2 X o/n incubation at RT; ABC amplification). Strong nuclear staining observed in spinal cord [A] 20x objective, [B] 40x objective. Staining performed in free floating IHC on 30µm saggital spinal cord sections. Find detailed information regarding the protocol in the Specific Protocol link.

    References for Anti-CREB antibody (ab11924)

    ab11924 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab11924.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"