Overview

  • Product name
  • Description
    Rabbit polyclonal to CREB
  • Tested applications
    Suitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 6 - 23 of Human CREB.

  • Positive control
    • A-431 nuclear extract.
  • General notes
    Keep on ice when not in storage.

Properties

Applications

Our Abpromise guarantee covers the use of ab11924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
WB
  • Application notes
    IHC-Fo: 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 39 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
    • Involvement in disease
      Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
    • Sequence similarities
      Belongs to the bZIP family.
      Contains 1 bZIP domain.
      Contains 1 KID (kinase-inducible) domain.
    • Post-translational
      modifications
      Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Active transcription factor CREB antibody
      • cAMP response element binding protein 1 antibody
      • cAMP response element binding protein antibody
      • cAMP responsive element binding protein 1 antibody
      • cAMP-responsive element-binding protein 1 antibody
      • CREB antibody
      • CREB-1 antibody
      • CREB1 antibody
      • CREB1_HUMAN antibody
      • Cyclic AMP-responsive element-binding protein 1 antibody
      • MGC9284 antibody
      • OTTHUMP00000163864 antibody
      • OTTHUMP00000163865 antibody
      • OTTHUMP00000206660 antibody
      • OTTHUMP00000206662 antibody
      • OTTHUMP00000206667 antibody
      • Transactivator protein antibody
      see all

    Images



    • Predicted band size : 39 kDa


      ab11924 at 1/1000 dilution staining Human CREB in A431 cells (nuclear extract) by Western blot (ECL).
    • CREB antibody (ab11924) at a dilution of 1/100 (2 X o/n incubation at RT; ABC amplification). Strong nuclear staining observed in spinal cord [A] 20x objective, [B] 40x objective. Staining performed in free floating IHC on 30µm saggital spinal cord sections. Find detailed information regarding the protocol in the Specific Protocol link.

    References

    This product has been referenced in:
    • Kammerer S  et al. Critical evaluation of KCNJ3 gene product detection in human breast cancer: mRNA in situ hybridisation is superior to immunohistochemistry. J Clin Pathol 69:1116-1121 (2016). ICC ; Human . Read more (PubMed: 27698251) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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