Overview

  • Product nameAnti-CREB antibody - ChIP GradeSee all CREB primary antibodies ...
  • Description
    Rabbit polyclonal to CREB - ChIP Grade
  • SpecificityImmunogen has 92% alignment to ATF-1
  • Tested applicationsChIP, WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) - non-phosphopeptide derived from human CREB around the phosphorylation site of Serine 133.

  • Positive control
    • HeLa cell extract. Human breast carcinoma. This antibody gave a positive result when used in the following methanol fixed cell lines: A549

Properties

Applications

Our Abpromise guarantee covers the use of ab31387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 20622856
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionThis protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
  • Involvement in diseaseDefects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
  • Sequence similaritiesBelongs to the bZIP family.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • Post-translational
    modifications
    Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Active transcription factor CREB antibody
    • cAMP response element binding protein 1 antibody
    • cAMP response element binding protein antibody
    • cAMP responsive element binding protein 1 antibody
    • cAMP-responsive element-binding protein 1 antibody
    • CREB 1 antibody
    • CREB antibody
    • CREB-1 antibody
    • CREB1 antibody
    • CREB1_HUMAN antibody
    • Cyclic AMP-responsive element-binding protein 1 antibody
    • MGC9284 antibody
    • OTTHUMP00000163864 antibody
    • OTTHUMP00000163865 antibody
    • OTTHUMP00000206660 antibody
    • OTTHUMP00000206662 antibody
    • OTTHUMP00000206667 antibody
    • Transactivator protein antibody
    see all

Anti-CREB antibody - ChIP Grade images

  • ICC/IF image of ab31387 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31387 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CREB antibody - ChIP Grade (ab31387) at 1/1000 dilution

    Lane 1 : Mouse primary hippocampal neurons whole cell lysate
    Lane 2 : Rat primary hippocampal neurons whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    Undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 ab31387. Left: Untreated sample; Right: Sample treated with synthesized peptide (negative control).Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 ab31387. Left: Untreated sample; Right: Sample treated with synthesized peptide (negative control).
  • Lane 1 : Anti-CREB antibody - ChIP Grade (ab31387) at 1/500 dilution
    Lane 2 : Anti-CREB antibody - ChIP Grade (ab31387) at 1/500 dilution (treated with synthesized peptide )

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract


    Predicted band size : 37 kDa

References for Anti-CREB antibody - ChIP Grade (ab31387)

This product has been referenced in:
  • Vialou V  et al. Serum response factor and cAMP response element binding protein are both required for cocaine induction of ?FosB. J Neurosci 32:7577-84 (2012). ChIP ; Mouse . Read more (PubMed: 22649236) »
  • Gao J  et al. A novel pathway regulates memory and plasticity via SIRT1 and miR-134. Nature 466:1105-9 (2010). WB, ChIP ; Mouse . Read more (PubMed: 20622856) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Rat Cell lysate - whole cell (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

Submitted Oct 14 2013

Application ChIP
Sample Human Cell lysate - whole cell (Ovarian carcinoma)
Specification Ovarian carcinoma
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Taqman
Positive control CREB binding site in the FOS promoter
Negative control 5' UTR of gene of interest (no CREB binding sites)
Username

Abcam user community

Verified customer

Submitted Jan 21 2012

Thank you for contacting us. Please find the requested MSDS documents attached. Please let me know if you need any additional information.

Thank you for contacting us. I have asked the lab  what they consider a positive ChIP result. I think they will want to know how you calculated the 0.09 - fold enrichment. You also mentiioned a 1.5 - fold enrichment for a positive control. Can you tell...

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Gracias por ponerse en contacto con nosotros. Hemos llevado a cabo un alineamiento de secuencia mediante BLAST, y existe un 92% de coincidencia con ATF-1. Aunque nosotros no lo hemos testado específicamente en el laboratorio, es muy posible que ...

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