Synthetic peptide (Human) - non-phosphopeptide derived from human CREB around the phosphorylation site of Serine 133.
Our Abpromise guarantee covers the use of ab31387 in the following tested applications.
|ChIP||Use at an assay dependent concentration. PubMed: 20622856|
|WB||1/500 - 1/1000. Predicted molecular weight: 37 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ICC/IF image of ab31387 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31387 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image is courtesy of an anonymous Abreview