Anti-CREB (phospho S121) antibody (ab14830)

Overview

  • Product nameAnti-CREB (phospho S121) antibody
    See all CREB primary antibodies
  • Description
    Rabbit polyclonal to CREB (phospho S121)
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Phospho-peptide spanning the Ser-121 phosphorylation site of human CREB.

Properties

Applications

Our Abpromise guarantee covers the use of ab14830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 37 kDa.
IP Use a concentration of 0.5 µg/ml.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionThis protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
  • Involvement in diseaseDefects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
  • Sequence similaritiesBelongs to the bZIP family.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • Post-translational
    modifications
    Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Active transcription factor CREB antibody
    • cAMP response element binding protein 1 antibody
    • cAMP response element binding protein antibody
    • cAMP responsive element binding protein 1 antibody
    • cAMP-responsive element-binding protein 1 antibody
    • CREB antibody
    • CREB-1 antibody
    • CREB1 antibody
    • CREB1_HUMAN antibody
    • Cyclic AMP-responsive element-binding protein 1 antibody
    • MGC9284 antibody
    • OTTHUMP00000163864 antibody
    • OTTHUMP00000163865 antibody
    • OTTHUMP00000206660 antibody
    • OTTHUMP00000206662 antibody
    • OTTHUMP00000206667 antibody
    • Transactivator protein antibody
    see all

Anti-CREB (phospho S121) antibody images

  • Immunofluorescent staining for CREB (phospho S121) obtained with ab14380 antibody in rat brain cortex. Picture taken with X10 objective. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT.  Protocol : free floating IHC on 30um cryostat sections. Primary antibody ab14830 was used at 1/500 and incubated overnight at room temperature. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2 hours at room temperature.

    Immunofluorescent staining for CREB (phospho S121) obtained with ab14380 antibody in rat brain cortex. Picture taken with X10 objective. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT.  Protocol : free floating IHC on 30um cryostat sections. Primary antibody ab14830 was used at 1/500 and incubated overnight at room temperature. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2 hours at room temperature.

References for Anti-CREB (phospho S121) antibody (ab14830)

This product has been referenced in:
  • Okoshi R  et al. Transcriptional regulation of tumor suppressor p53 by cAMP-responsive element-binding protein/AMP-activated protein kinase complex in response to glucose deprivation. Genes Cells 14:1429-40 (2009). Read more (PubMed: 19930465) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (glioblastoma)
Loading amount 50 µg
Specification glioblastoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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Abcam user community

Verified customer

Submitted Nov 27 2006

Thank you for your enquiry. I am sorry that my response has taken so long an answer has not been forthcoming form the lab. The only antibody that I have details of where it has been tested for reactivity against CREM and ATF is ab30651. This antibod...

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Thank you for your enquiry. We do not recommend a specific protocol for western blotting for ab14830, you should be able to use it with a standard WB protocol. I would like to emphasise if you do not have experience of WB for phospho-proteins to be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"