Anti-CREB (phospho S129 + S133) antibody (ab10564)


  • Product nameAnti-CREB (phospho S129 + S133) antibody
    See all CREB primary antibodies
  • Description
    Rabbit polyclonal to CREB (phospho S129 + S133)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (Human).

  • Positive control
    • NIH3T3 cells with and without PDGF stimulation; Y-1 cells.



Our Abpromise guarantee covers the use of ab10564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).


  • FunctionThis protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
  • Involvement in diseaseDefects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
  • Sequence similaritiesBelongs to the bZIP family.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • Post-translational
    Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Active transcription factor CREB antibody
    • cAMP response element binding protein 1 antibody
    • cAMP response element binding protein antibody
    • cAMP responsive element binding protein 1 antibody
    • cAMP-responsive element-binding protein 1 antibody
    • CREB antibody
    • CREB-1 antibody
    • CREB1 antibody
    • CREB1_HUMAN antibody
    • Cyclic AMP-responsive element-binding protein 1 antibody
    • MGC9284 antibody
    • OTTHUMP00000163864 antibody
    • OTTHUMP00000163865 antibody
    • OTTHUMP00000206660 antibody
    • OTTHUMP00000206662 antibody
    • OTTHUMP00000206667 antibody
    • Transactivator protein antibody
    see all

Anti-CREB (phospho S129 + S133) antibody images

  • Predicted band size : 37 kDa
    TPeptide Competition and Phosphatase Treatment: Lysates prepared from NIH3T3 cells stimulated with PDGF were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab10564 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the chemilumiescence. The data show that the peptide corresponding to ab10564 blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific. TPeptide Competition and
  • Immunohistochemistry (PFA perfusion fixed frozen section) staining of CREB (phospho S129 + S133) in the mouse cortex. Samples were fixed with paraformaldehyde and ab10564 was used at 1/1000 for 18 hours at 25ºC. An Alexa Fluor®568 conjugated donkey anti-Rabbit secondary was used at 1/1000.

    See Abreview

References for Anti-CREB (phospho S129 + S133) antibody (ab10564)

This product has been referenced in:
  • Su Y  et al. Receptor desensitization and blockade of the suppressive effects of prostaglandin E(2) and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes. Cancer Immunol Immunother 60:111-22 (2011). WB ; Human . Read more (PubMed: 20960188) »
  • Harmon EB  et al. Beta-adrenergic receptor activation in immortalized human urothelial cells stimulates inflammatory responses by PKA-independent mechanisms. Cell Commun Signal 3:10 (2005). WB . Read more (PubMed: 16091148) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 150 µg
Gel Running Conditions Reduced Denaturing (gel 10% SDS-PAGE)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Treatment Forskoline 1mg/kg i.p.
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Karine Thibault

Verified customer

Submitted Nov 04 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde

Dr. Karine Thibault

Verified customer

Submitted Aug 19 2014

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

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Thank you for getting back to me. Yes, it seems that this product might be binding specifically. In which case, I am more than happy to offer you a replacement vial of your choice, ab47373. Please confirm your shipping address/purchasing agent so th...

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Thank you for your patience. I am sorry to hear that you are experiencing difficulties with this product ab10564 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the...

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Thank you for your enquiry. I am sorry that my response has taken so long an answer has not been forthcoming form the lab. The only antibody that I have details of where it has been tested for reactivity against CREM and ATF is ab30651. This antibod...

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Thank you for your enquiry. To our knowledge this antibody has yet to be tested for cross reactivity with pS133-CREB. At the moment, all the information we have available is that which is listed on the datasheet. If you choose to test this antibody for...

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