Overview

  • Product nameAnti-CREB3 antibody
    See all CREB3 primary antibodies
  • Description
    Rabbit polyclonal to CREB3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: YSSDTRGSLP AEHGVLSRQL RALPSEDPYQ LELPALQSEV PKDSTHQWLD , corresponding to Internal sequence amino acids 247-296 of Human CREB3

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab42454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Target

  • RelevanceThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element, an octameric palindrome. The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. An additional transcript variant has been identified, but its biological validity has not been determined.
  • Cellular localizationIsoform 2: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Membrane. Isoform 3: Nucleus. Cytoplasm.
  • Database links
  • Alternative names
    • Basic leucine zipper protein antibody
    • cAMP responsive element binding protein 3 antibody
    • Clic AMP responsive element binding protein 3 antibody
    • CREB 3 antibody
    • cyclic AMP response element (CRE) binding protein/activating transcription factor 1 antibody
    • Cyclic AMP responsive element binding protein 3 antibody
    • Leucine zipper protein antibody
    • LUMAN antibody
    • LZIP antibody
    • MGC15333 antibody
    • MGC19782 antibody
    • Transcription factor LZIP alpha antibody
    see all

Anti-CREB3 antibody images

  • All lanes : Anti-CREB3 antibody (ab42454) at 0.5 µg/ml

    Lane 1 : MW Markers
    Lane 2 : Jurkat Cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CREB3 antibody (ab42454)

ab42454 has not yet been referenced specifically in any publications.

Product Wall




I don't expect any problems with the ab4570 as this one works fine in IP as well, however, I am not sure about the ab42454.

Nonetheless, I am not too concerned either: ab42454 is not tested in IP, but it works in ELISA, which is...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"