Anti-CREB3L1 antibody (ab112021)

Overview

  • Product name
  • Description
    Goat polyclonal to CREB3L1
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    C-DFSHSKEWFHDRD

    , corresponding to C terminal amino acids 497-509 of Human CREB3L1.

  • Positive control
    • Human Liver and Human Skeletal Muscle tissue; A549 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab112021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 3.75 µg/ml.
ELISA 1/32000.

Target

  • Function
    Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). May play a role in gliosis. In vitro, binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE.
  • Tissue specificity
    Ubiquitously expressed with high levels in pancreas and prostate. Expressed at relatively lower levels in brain.
  • Sequence similarities
    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications
    Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is performed sequentially by site-1 and site-2 proteases (PS1 and PS2) and is triggered by translocation to the Golgi apparatus.
  • Cellular localization
    Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BBF-2 homolog antibody
    • CAMP responsive element binding protein 3 like 1 antibody
    • cAMP-responsive element-binding protein 3-like protein 1 antibody
    • CR3L1_HUMAN antibody
    • Creb3l1 antibody
    • Cyclic AMP responsive element binding protein 3 like protein 1 antibody
    • OASIS antibody
    • Old astrocyte specifically-induced substance antibody
    • Processed cyclic AMP-responsive element-binding protein 3-like protein 1 antibody
    see all

Images

  • Immunohistochemical analysis of CREB3L1 expression in formalin-fixed, paraffin-embedded Human liver using ab112021 at 3.75 µg/ml.
  • Immunohistochemical analysis of CREB3L1 expression in formalin-fixed, paraffin-embedded Human skeletal muscle using ab112021 at 3.75 µg/ml.
  • Anti-CREB3L1 antibody (ab112021) at 3 µg/ml + A549 cell lysate

    Predicted band size : 57 kDa

References

ab112021 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (SAOS-2, U2OS, HEK 293T, ATDC5)
Loading amount
15 µg
Specification
SAOS-2, U2OS, HEK 293T, ATDC5
Treatment
0.02 ug/ml tunicamycin for 72h
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

Submitted Dec 15 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human vascular smooth muscle cells)
Specification
human vascular smooth muscle cells
Fixative
Methanol/Acetone
Permeabilization
Yes - 0.5% NP-40
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

Submitted Nov 30 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vector Labs Unmasking Solution H-3300
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

Submitted Nov 30 2011

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab33051 with the order number. This is currently out of stock, but you can expect ...

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Thank you for your reply. You can see the WB data on our online datasheet. http://www.abcam.com/CREB3L1-antibody-ab112021.pdf Please let me know if the protocol suggestions do not improve your results.  I look forward to your reply.

Thank you for your reply and for sending your protocol and images. There are a few adjustments to the protocol that you may want to try. For nuclear proteins, these can sometimes be difficult to extract.  We usually recommend the addition of SDS fo...

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