The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
1/50 - 1/100.
FunctionTranscription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). May play a role in gliosis. In vitro, binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE.
Tissue specificityUbiquitously expressed with high levels in pancreas and prostate. Expressed at relatively lower levels in brain.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain.
Post-translational modificationsControlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is performed sequentially by site-1 and site-2 proteases (PS1 and PS2) and is triggered by translocation to the Golgi apparatus.
Cellular localizationEndoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.