Overview

  • Product nameAnti-CREB3L2 antibody
    See all CREB3L2 primary antibodies
  • Description
    Rabbit polyclonal to CREB3L2
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 491-520 of Human CREB3L2

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab102989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 57 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionTranscriptional activator which is processed and activated during endoplasmic reticulum stress late phase. Binds to the cAMP response element (CRE) and activates transcription through CRE. Regulates the transcription of unfolded protein response target genes, preventing accumulation of unfolded proteins in damaged neurons. Also regulates the expression of SEC23A, accelerating protein trafficking from the ER to the Golgi thereby playing a key role in chondrocyte differentiation and formation of epiphyseal cartilage.
  • Tissue specificityWidely expressed with highest levels in placenta, lung, spleen and intestine, and lowest levels in heart, brain, skeletal muscle, thymus, colon and leukocytes. In fetal tissues, the weakest expression is detected in brain and heart.
  • Involvement in diseaseA chromosomal aberration involving CREB3L2 is found in low grade fibromyxoid sarcoma (LGFMS). Translocation t(7;16)(q33;p11) with FUS.
  • Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    modifications
    Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1 and PS2).
  • Cellular localizationEndoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • B ZIB transcription factor antibody
    • Basic transcription factor 2 antibody
    • BBF2 human homolog on chromosome 7 antibody
    • BBF2H7 antibody
    • BZIB transcription factor antibody
    • cAMP responsive element binding protein 3 like 2 antibody
    • cAMP-responsive element-binding protein 3-like protein 2 antibody
    • CR3L2_HUMAN antibody
    • Creb3l2 antibody
    • Cyclic AMP-responsive element-binding protein 3-like protein 2 antibody
    • FUS/BBF2H7 protein antibody
    • MGC131709 antibody
    • MGC71006 antibody
    • Processed cyclic AMP-responsive element-binding protein 3-like protein 2 antibody
    • TCAG_1951439 antibody
    see all

Anti-CREB3L2 antibody images

  • Anti-CREB3L2 antibody (ab102989) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 57 kDa
  • ab102989 staining CREB3L2 in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1.5% serum for 1 hour at 25°C and then incubated with ab102989 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an undiluted biotin conjugated goat polyclonal.

    See Abreview

  • Immunofluorescence analysis of U2-OS, staining CREB3L2 with ab102989.

    Cells were fixed with formaldehyde, permeabilized with 0.5% NP-40 in PBS and blocked with 3% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 3% BSA in PBS) for 12 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-CREB3L2 antibody (ab102989)

ab102989 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Specification U2OS
Fixative Formaldehyde
Permeabilization Yes - 0.5% NP40/PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 25 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (primary vascular smooth muscle cells)
Specification primary vascular smooth muscle cells
Fixative Methanol/Acetone
Permeabilization Yes - 0.5% NP-40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

Submitted Mar 14 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Vector Labs Unmasking Solution H-3300
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

Submitted Mar 14 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SAOS-2, U2OS, HEK 293T, ATDC5)
Loading amount 15 µg
Specification SAOS-2, U2OS, HEK 293T, ATDC5
Treatment 0.02 ug/ml tunicamycin for 72h
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

Submitted Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"