Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab76856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional activator which is processed and activated during endoplasmic reticulum stress late phase. Binds to the cAMP response element (CRE) and activates transcription through CRE. Regulates the transcription of unfolded protein response target genes, preventing accumulation of unfolded proteins in damaged neurons. Also regulates the expression of SEC23A, accelerating protein trafficking from the ER to the Golgi thereby playing a key role in chondrocyte differentiation and formation of epiphyseal cartilage.
    • Tissue specificityWidely expressed with highest levels in placenta, lung, spleen and intestine, and lowest levels in heart, brain, skeletal muscle, thymus, colon and leukocytes. In fetal tissues, the weakest expression is detected in brain and heart.
    • Involvement in diseaseA chromosomal aberration involving CREB3L2 is found in low grade fibromyxoid sarcoma (LGFMS). Translocation t(7;16)(q33;p11) with FUS.
    • Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP (basic-leucine zipper) domain.
    • Post-translational
      modifications
      Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1 and PS2).
    • Cellular localizationEndoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • B ZIB transcription factor antibody
      • Basic transcription factor 2 antibody
      • BBF2 human homolog on chromosome 7 antibody
      • BBF2H7 antibody
      • BZIB transcription factor antibody
      • cAMP responsive element binding protein 3 like 2 antibody
      • cAMP-responsive element-binding protein 3-like protein 2 antibody
      • CR3L2_HUMAN antibody
      • Creb3l2 antibody
      • Cyclic AMP-responsive element-binding protein 3-like protein 2 antibody
      • FUS/BBF2H7 protein antibody
      • MGC131709 antibody
      • MGC71006 antibody
      • Processed cyclic AMP-responsive element-binding protein 3-like protein 2 antibody
      • TCAG_1951439 antibody
      see all

    Anti-CREB3L2 antibody images

    • Anti-CREB3L2 antibody (ab76856) at 5 µg/ml + recombinant tagged fragment (immunogen) at 0.2 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 57 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    References for Anti-CREB3L2 antibody (ab76856)

    This product has been referenced in:
    • Imamura K  et al. Human immunodeficiency virus type 1 enhancer-binding protein 3 is essential for the expression of asparagine-linked glycosylation 2 in the regulation of osteoblast and chondrocyte differentiation. J Biol Chem 289:9865-79 (2014). WB . Read more (PubMed: 24563464) »

    See 1 Publication for this product

    Product Wall

    Thank you for submitting an Abreview of ab76856. As you may have noticed, your review has now been published on our website.

    Since you obtained poor results using the antibody in a untested species and application, we would like to follow up...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Liver)
    Specification Liver
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Vector Labs Unmasking Solution H-3300
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
    Username

    Leilani Beltran

    Verified customer

    Submitted Mar 21 2012

    Thank you for submitting your Abreview of ab76856. I am sorry to hear it did not work as expected. If you have purchased this antibody within the past 6 months or so I would be happy to offer a replacement, credit or refund. Please include the purchase...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (primary vascular smooth muscle cells)
    Specification primary vascular smooth muscle cells
    Fixative Methanol/Acetone
    Permeabilization Yes - 0.5% NP-40
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Leilani Beltran

    Verified customer

    Submitted Dec 01 2011

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (SAOS-2, U2OS, HEK 293T, ATDC5)
    Loading amount 15 µg
    Specification SAOS-2, U2OS, HEK 293T, ATDC5
    Treatment 0.02 ug/ml tunicamycin for 72h
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Leilani Beltran

    Verified customer

    Submitted Nov 30 2011

    Thank you for your reply. I have processed your request for a replacement antibody, ab102989. If there is anything else I can do to help, please let me know.

    I would be happy to a replacement CREB3L2 antibody. The only other one we have in our catalogue is ab102989, would this one be suitable for you? Also to process the replacement, could you please provide me with the original order number?

    Thank you for contacting Abcam and providing your protocol and western blot image. I have looked over your protocol and the image that you sent. Your protocol looks to be fine and you have tried multiple variations to resolve this issue. For the weste...

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    I am sorry that you are experiencing problems with this antibody in western blot. According to the data sheet, this antibody has only been tested against the recombinant fragment used as immunogen and has not been tested on endogenous protein levels. ...

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    Predicted molecular weight of the recombinant fragment (immunogen sequence plus 26kDa tag) is 37kDa. This is what is shown on the WB images on the datasheet. The predicted size of the protein CREB3L2 is taken from information of the swissprot s...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"