The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionActs as a negative regulator of the endoplasmic reticulum stress response or unfolded protein response (UPR). Represses the transcriptional activity of CREB3 during the UPR. Recruits CREB3 into nuclear foci.
Sequence similaritiesBelongs to the bZIP family. CREBRF subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Post-translational modificationsProbably degraded by the proteasome.
Cellular localizationNucleus. Colocalizes with CREB3 in nuclear foci.