Overview

  • Product nameAnti-CREM antibody
    See all CREM primary antibodies
  • Description
    Mouse polyclonal to CREM
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse
  • Immunogen

    Fusion protein:

    MTMETVESQQDRSVTHSVAEHSSLHMQTGQISVPTLAQVSVAGSGTGRGS PAVTLVQLPSGQTVQVQGVIQTPHPSVIQSPQIQTVQVATIAETDDSADS

    , corresponding to amino acids 1-100 of Rat CREM.

  • General notesProduced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferConstituents: 50% Glycerol
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 32 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionTranscriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.
  • Tissue specificityExpressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
  • Sequence similaritiesBelongs to the bZIP family.
    Contains 1 bZIP (basic-leucine zipper) domain.
    Contains 1 KID (kinase-inducible) domain.
  • Post-translational
    modifications
    Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
    Stimulated by phosphorylation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP response element modulator antibody
    • cAMP responsive element modulator antibody
    • cAMP-responsive element modulator antibody
    • CREM antibody
    • CREM-2 antibody
    • CREM_HUMAN antibody
    • hCREM 2 antibody
    • hCREM-2 antibody
    • hCREM2 antibody
    • ICER antibody
    • Inducible cAMP early repressor antibody
    • Inducible cAMP early repressor ICER antibody
    • MGC111110 antibody
    • MGC17881 antibody
    • MGC41893 antibody
    see all

References for Anti-CREM antibody (ab21763)

ab21763 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"