The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21383078
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 37 kDa).
Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.
Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
Belongs to the bZIP family. Contains 1 bZIP (basic-leucine zipper) domain. Contains 1 KID (kinase-inducible) domain.
Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome. Stimulated by phosphorylation.